Streptococcus pneumoniae obtained from carriers: risk factors for colonization, serotypes and effect of 10-valent pneumoccocal conjugate vaccine.
Federal University of Health Sciences of Porto Alegre (UFCSPA)
Basic Health Sciences Department
Professor Marilene Porawski Garrido, PhD
Professor Cicero Dias, PhD
English, Portuguese
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
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Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Streptococcus pneumoniae is among the most important human pathogens, being responsible for invasive disease, with a high case-fatality rate. Besides this, this agent is responsible for about 50% of community-acquired pneumonia. Children and the elderly are the groups at highest risk for invasive infections. Since the year 2010, a conjugate-vaccine, including the 10 most common serotypes (PCV-10) is applied to children in order to prevent invasive disease and its consequences. Changes in the prevalence of serotypes are expected due to selective pressure due to the vaccine. Adults, especially the elderly, may have and indirect benefit of the vaccine, since the nasopharynx colonization is eliminated and the transmission from children does not occur.
What is the aim of the project?
To define the effect of 10-valent ´pneumococcal conjugate vaccine on the prevalence of Streptococcus pneumoniae serotypes among carriers (children and adult).
What techniques and methods are used?
Samples from the nasopharynx of children and the elderly will be obtained and submitted to culture. Suspected colonies will be evaluated to confirm the presence of the agent. Isolates will be serotyped by PCR (Polymerase Chain Reaction) or Quellung reaction.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student is expected to follow the process of sample collection and to perform laboratory procedures. These include cultures on sheep Blood Agar plates with incubation in CO2.After 24 hours of incubation, plates will be inspected for the presence of suspected colonies; with further confirmation by biochemical and physiological tests (optochin susceptibility and bile solubility). Isolates with identification confirmed as S. pneumoniae will be further submitted to serotyped by either PCR or Quellung reaction. Epidemiological data of the subjects will be evaluated in terms of potential risk factors for colonization, and association of serotypes.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
The student will be exposed to theoretical activities such as readings, lectures and seminars.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Basic laboratory skills.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- Pre-Medical students from the American-British system
- Students in biomedical fields
- MOTT; MARIANA ; Caierão; Juliana ; ROSA DA CUNHA; GABRIELA ; RODRIGUES PEREZ; LEANDRO REUS ; MATUSIAK; ROBERTO ; PILGER DE OLIVEIRA; KATIA RUSCHEL ; D?AZEVEDO; PEDRO ALVES ; DIAS; CÍCERO . Susceptibility profiles and correlation with pneumococcal serotypes soon after implementation of the 10-valent pneumococcal conjugate vaccine in Brazil. International Journal of Infectious Diseases; v. 20; p. 47-51; 2013.
- Caierão; Juliana ; HAWKINS; PAULINA ; SANT?ANNA; FERNANDO HAYASHI ; DA CUNHA; GABRIELA ROSA ; D?AZEVEDO; PEDRO ALVES ; MCGEE; LESLEY ; DIAS; CÍCERO . Serotypes and Genotypes of Invasive Streptococcus pneumoniae Before and After PCV10 Implementation in Southern Brazil. Plos One; v. 9; p. e111129; 2014.