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Determination of synaptic vesicle pools in hippocampal neurons in culture
Spain (IFMSA Spain) - University of Sevilla, Sevilla
Biophysics and Medical Physics
Physiology and Biophysics
Juan José Toledo Aral
Guillermo Álvarez de Toledo
Type of Research Project
- Basic science
What is the background of the project?
Synaptic transmission is the key cellular process that determines neuronal communication and basically all brain functions. Any alteration in synaptic transmission leads to disease, including Amiotrophyc Lateral Sclerosis (ALS), Spinal muscular atrophy (AMS) or neurodegenerative disorders like Alzheimer or Parkinson disease. The quantification of synaptic vesicles in neurons is of fundamental importance to know when disease will appear. In our lab we use hippocampal neurons in culture and measure neuronal activity with genetically encoded fluorescence proteins in an inverted microscope. We also use mice carrying different mutation of proteins involved in synaptic transmission.
What is the aim of the project?
To quantify the number of synaptic vesicles pools that are mobilized during brain activity
What techniques and methods are used?
Neuronal primary cultures Electrophysiology Image processing and analysis Genetically encoded fluorescence proteins
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
1. Learn to use a primary culture room 2. Dissection of newborn mice 3. Protocol of neuronal primary cultures 4. Participate in data collection in a fluorescence microscope 5. Participate in the preparation of solutions required for the experiments 6. Analysis of imaging data 7. Preparation of figures for publication 8. Preparation of a manuscript for publication 9. Bibliography search and organization
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Explanations will be done as the student perform his/her task
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation - The student will prepare a scientific report - The student’s name will be mentioned in a future publication
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Motivation, dedication and enthusiasm
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Students in biomedical fields
- Segovia; M.; Alés; E. Montes; Bonifás; I.; Jemal; I. M.A.;Lindau; M. Maximov; A.; Südhof; T.C. and Alvarez de Toledo; G. 2010. Push and pull regulation of the fusion pore by synaptotagmin-7. Proc Natl Acad Sci U S A. 2010;107:19032-7.
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