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IMPLEMENTATION OF ALTERNATIVE RESEARCH METHODS ON THE USE OF ANIMAL MODELS IN TOXICOLOGY AND QUALITY CONTROL OF MEDICAL DEVICES.
University of Costa Rica
Laboratory of Biological Trials.
Dra. Sara González Camacho
Dra. Sara González Camacho
English and Spanish
Type of Research Project
- Basic science
What is the background of the project?
In the international level, the use of animal models on preclinical research hasn't found a substitution. This is because hasn't been develop alternative methods, that could evidence the systemic response of organisms through exposition on differents chemical compounds, biological and biotechnological. For this reason, has been an uprise in the growing of new methodologies in vitro that could complement the toxicological studies, or otherwise substitute some methodologies of quality control of drugs for human and veterinary use. (Repetto and Zurita, 2012). The evaluation of safety and efficacy of compounds that will be used as pesticides, drugs, cosmetics and other compounds, has push the development of alternative methods to the use of animal as research models in investigation, among them the use of nanotechnology in the biomedical area (Olazabal 2015), also the use of cell lines in toxicology trials. This methods has been going in continuing progress and search of other possibilities that could improve the work of researches, and the fulfillment of the ethical, political and legal reasons on the use of animals in biomedical research.
What is the aim of the project?
The present activity has as objective the implementation of a series of trials in vitro used as alternative methods at the use of animals, complementing the preclinical research that is on going in the Biological Tests Laboratory in the toxicology area and quality control of drugs and medical supplies, also the investigation on the effect of the new materials used in the production of medical devices, as well of new active chemical compounds coming from medicinal plants, given by nanoparticles in the diana tisue. For this purpose it has been standardized different techniques based on international guidelines such as OECD (Guidelines chemicals section 4), ISO 10993 and USP ( United States Pharmacopeia) in the latest version.
What techniques and methods are used?
● USP 38-NF-(87) Biological reactivity test, in vitro trial of diffusion in agar ● Direct contact trial ● Elution trial ● OECD Genetic toxicology guidance document, 2015 micronucleus trial ● Cytotoxicity trisl ● Statistical analysis
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
- To perform the experimental techniques mentioned above. - To analyze and interpret experimental data. - To prepare a scientific report. - To prepare a presentation to the research group.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
1- Yes. Induction in modules for the correct use of laboratory animals.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster - The student will prepare a presentation
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Requirements Third years of medicine and to be able to work in group. Subjects passed: Physiology, biochemistry, microbiology, parasitology and medical genetics. Previous experience with: Microscopy basic and basic laboratory
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields
- 1- Meneau. R (2014) Métodos Alternativos en Toxicología Habana; Cuba. Volumen 45; Número 1. Recuperado de http://revista.cnic.edu.cu/revistaCB/articulos/m%C3%A9todos-alternativos-en-toxicolog%C3%ADa.
- 2- <http://lebi.ucr.ac.cr/index.php/es/>
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