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A new intracellular monitoring method for Calcinurin-immunosuppressed patients after kidney transplantation
Germany (bvmd) - Universitaet Duisburg-Essen, Essen
Institut für Transfusionsmedizin, Robert-Koch-Haus, Universitätsklinikum Essen, Virchowstraße 179. 45147 Essen, Germany
Prof. Dr. med. Peter Horn
PD Dr. rer. medic. Vera Rebmann
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
After kidney transplantation patients are regularily immunosuppressed with Calcinurininhibitors e.g. Ciclosporin A or Tacrolimus. Patients are currently monitored by checking the serum level of the corresponding immunosuppressant. Sometimes dosages are chosen too high or too low. We are trying to establish a new intracellular monitoring method checking for NFkB (nuclear factor kappa-light-chain-enhancer of activated B cells) translocation in order to provide better treatment for transplanted patients.
What is the aim of the project?
We are trying to establish a new monitoring method for immunosuppression in kidney transplanted patients.
What techniques and methods are used?
Methods used: ● sterile isolation of peripheral blood cells ● stimulation of blood cells ● dying of surface proteins and intracellular transcription factors ● image flow cytometry (Amnis) In order to find a monitoring method checking for NFkB translocation.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
The student is expected to be motivated and interested in learning about the immunosuppression in kidney transplanted patients. He/she will use sterile isolation of peripheral blood cells of kidney transplanted patients and then stimulate these blood cells with different immunosuppressant. Due to the dosages he/she will monitor the dying of surface proteins and intracellular transcription factors via image flow cytometry. All these tasks will be firstly done under supervision and he/she will able to do it on his/her own, the student might do it on his/her own.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report - The student’s name will be mentioned in a future publication
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
We are willing to accept a motivated student with basic knowledge in immunology to support our research team. We request a short skype interview before accepting a new student. We are willing to accept a motivated student with basic knowledge in immunology to support our research team. Subjects passed: Cell Biology, Molecular Biology Previous experience with: Basic Lab work
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Students in biomedical fields
- The Ca2+-dependent Phosphatase Calcineurin Controls the Formation of the Carma1-Bcl10-Malt1 Complex during T Cell Receptor-induced NF- B Activation - Brunner; Andrea Eitelhuber; Daniel Lysann Palkowitsch; Uta Marienfeld; Cornelia; doi: 10.1074/jbc.M110.155895 originally published online January 3; 2011
- Nuclear Translocation of Nuclear Factor of Activated T Cells (NFAT) as a Quantitative Pharmacodynamic Parameter for Tacrolimus - Orla Maguire; Kathleen M. Tornatore; Kieran L. O’Loughlin; Rocco C. Venuto; Hans Minderman
- Simultaneous assessment of NF-κB/p65 phosphorylation and nuclear localization using imaging flow cytometry - Orla Maguire; Kieran O'Loughlin; Hans Minderman
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