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Development of fluorescence probe for cancer detection
Thailand (IFMSA-Thailand) - Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok
Department of Immunology
Prof. Dr. Tararaj Dharakul
Assist. Prof. Dr. Peti Thuwajit
Type of Research Project
- Basic science
What is the background of the project?
Because of rapid growth in the field of oncology, we now have better standing in which marker gene and protein dominantly expressing in cancer. With several spectra, this lead to a novel promising technology in molecular imaging. Fluorescence protein probe has a benefit of its high sensitivity and specificity to target receptor. It hence allows researchers to study cancer in vivo such as tumor cell mobility, invasion, metastasis and angiogenesis. Choriocarcinoma rises from trophoblast cell at the interface between fetus and maternal circulation. It is rapidly invasive with early hematogenous metastasis. Some studies report a surface marker that specific to trophoblast cell such as Trop-1, Trop-2, Trop-3 and Trop-4. In addition, the invention of Bewo cell , the choriocarcinoma cell line, give rise to trophoblastic cell model which regularly use for in vitro study nowadays.
What is the aim of the project?
The aim of the project is to develop fluorescence-conjugated antibody probe for “Choriocarcinoma” detection. Synthetic protein probes specific to tumor antigens from Bewo cell will be screened, produced, and engineered to have signals as cancer detector. The products will be developed to detect cancer in clinical practice further.
What techniques and methods are used?
- Polymerase chain reaction (PCR) - Cloning - Plasmid Extraction - Protein purification - Cell culture - Western blot analysis - Dot blot analysis - Immunocytochemistry
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
The student should explain the overall project and objective of some experiments which are available at that time in the ongoing project. To illustrate, during your exchange you may have a chance to experience some molecular technique such as Protein purification, Polymerase chain reaction (PCR) and Western blot. The student should accept some responsibilities and laboratory duties; such as checking some instrumental maintenance daily or weekly. The student will join the academic activities such as lab meetings, journal clubs and seminars.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Student should have already studied the following subjects: Biology, Biochemistry and Molecular Biology.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields
- Kulkeaw L; Sakolvaree Y; Srimanote P; Tongtawe P; Maneewatch S; Sookrung N; Tungtrongchitr A; Tapchaisri P; Kurazano H; Chaicumpa W. Human monoclonal ScFv neutralize lethal Thai Cobra; Naja kaouthia; neurotoxin. J Proteomics. 2009 Mar 6;72(2):270-82. PubMed PMID: 19162253.
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