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(Talca) Role of cellular senescence in platelet aggregation
Universidad de Talca
Center for Medical Research, University of Talca School of Medicine
Nelson E. Brown
Spanish and english
Type of Research Project
- Basic science
What is the background of the project?
Disorders of platelet aggregation occur frequently in the context of ageing and age-related diseases such as cancer. While the functional connections between cancer cells and platelets have been well documented, little is known about the effects of senescent cells on platelet activation. Cellular senescence is a form of stable cell cycle arrest that is triggered by a variety of stimuli. Interestingly, in spite of their inability to proliferate, senescent cells are able to produce and secrete a plethora of factors that may promote extracellular matrix remodeling, or even stimulate proliferation of neighboring cells. Thus far, however, the effects of this Senescence-Associated Secretory Phenotype (SASP) on platelet function have not been explored. We surmise that components of the SASP might be able to modulate platelet function. What is the aim of the project? To determine the effect of factors secreted by senescent cells on platelet activity. What techniques and methods are used. Mention the steps/stages in this project? • Cell biology: tissue culture experiments, immunofluorescence, beta-Galactosidase staining (induction of senescence in vitro) • Molecular biology: Quantitative PCR (determination of SASP expression) • Platelet aggregation experiments (determination of the effect of SASP factors on platelets)
What is the aim of the project?
To determine the effect of factors secreted by senescent cells on platelet activity.
What techniques and methods are used?
Cell biology: tissue culture experiments, immunofluorescence, beta- Galactosidase staining (induction of senescence in vitro) Molecular biology: Quantitative PCR (determination of SASP expression) Platelet aggregation experiments (determination of the effect of SASP factors on platelets)
What is the role of the student?
What are the tasks expected to be accomplished by the student?
- Learn the basic techniques of molecular and cell biology that are necessary to answer the question he/she is interests in. - Present basic results to an audience of students and professors, and be able to explain the main problems he/she is facing. - General lab management. - Interact with other members of the lab.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
0No, all the teachings will be tutorial. Basic readings will be recomended during the development of the project.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student’s name will be mentioned in a future publication - The student will have the opportunity to present the results together with the supervisor at a conference
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Basic knowledge in molecular and cell biology. Subjects passed: Basic science curses (chemistry, biochemistry, molecular biology).
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system
- Franco AT; Corken A; Ware J. Platelets at the interface of thrombosis; inflammation; and cancer. Blood. 2015;126:582-8.
- Hanahan D; Weinberg Robert A. Hallmarks of Cancer: The Next Generation. Cell. 2011;144:646-74.
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