Projects
Name
Role of the bacterian translocation in the immune reconstitution and clinical resultas after an allogenic hematopoietic stem cell transplant
University
Catalonia (AECS) - Autonomous University of Barcelona (UAB) - Hospital Vall d'Hebron, Barcelona
Domain
Hematology
Departement
Experimental Hematology
Head
Francesc Bosch Albareda
Tutor
Marta Crespo Mauli
Languages
English
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No Yes Yes No No Yes Yes Yes Yes
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Allogeneic hematopoietic stem cell transplantation (allo-SCT) is the only curative treatment for many hematological diseases (mostly neoplasic) and some non-hematological (autoimmune, metabolic) diseases. However, the success of allo-SCT is conditioned by the ability to achieve a prolonged graft and a stable immune reconstitution, eradication of malignant or abnormal cells and a control of graft-versus-host disease (GVHD). GVHD occurs when allo-reactive donor T cells recognize patient antigens that have been exposed by cell damage caused by the conditioning treatment administered prior to the transplant. The study of markers related to bacterial translocation has great relevance in GVHD, since this phenomenon directly affects the cells of the immune system, altering the homeostasis by activating antigen-presenting cells and inducing the production of inflammatory cytokines that affect the mechanisms of immune tolerance of the host, which are key factors in both the development of GVHD and in the relapse of the patient's underlying disease.
What is the aim of the project? To correlate bacterial translocation markers with the immunoreactivity profile and clinical results after allogeneic hematopoietic stem cell transplantation.
What techniques and methods are used. Mention the steps/stages in this project? - Flow cytometry - ELISA (enzyme-linked immunosorbent assay)
Project stages: 1. To identify soluble markers of bacterial translocation as a prognostic factor of GVHD and leukemic relapse in plasma of patients in the peritransplantation period. 2. To analyze the immunophenotypic profile of the cells of the immune system of patients in the peritransplantation period and to correlate the different populations with the presence of soluble markers of bacterial translocation 3. To analyze the expression of PD-1, CTLA-4, LAG-3 immuno-checkpoints in patient regulatory T cells in the peritransplantation period and correlate it with the development of GVHD and leukemic relapse.
What is the aim of the project?
To correlate bacterial translocation markers with the immunoreactivity profile and clinical results after allogeneic hematopoietic stem cell transplantation.
What techniques and methods are used?
- Flow cytometry - ELISA (enzyme-linked immunosorbent assay) Project stages: 1. To identify soluble markers of bacterial translocation as a prognostic factor of GVHD and leukemic relapse in plasma of patients in the peritransplantation period. 2. To analyze the immunophenotypic profile of the cells of the immune system of patients in the peritransplantation period and to correlate the different populations with the presence of soluble markers of bacterial translocation. 3. To analyze the expression of PD-1, CTLA-4, LAG-3 immuno-checkpoints in patient regulatory T cells in the peritransplantation period and correlate it with the development of GVHD and leukemic relapse.
What is the role of the student?
What are the tasks expected to be accomplished by the student?
The student will look for and study bibliography about the different aspects of the project. The student will learn how to process primary samples from patients for the ELISA analysis and how to keep the information in a data base. The student will learn how to curate and analyze the data obtained. The student will also attend scientific seminars organized by our institution. The supervision will be tailored according to his/her performance.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
The student will be handed scientific reports to read and will attend institutional seminars.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Being fluent in English
Are there any legal limitations in the student’s involvement
No
Hours
8
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- Students in biomedical fields
Articles
- https://www.ncbi.nlm.nih.gov/pubmed/18832652
- https://www.ncbi.nlm.nih.gov/pubmed/20409584
- https://www.ncbi.nlm.nih.gov/pubmed/20557144