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Role of Sly adaptor proteins in the development of the immune system
Germany (bvmd) - Eberhard Karls Universitaet, Tuebingen
Institute of Pharmacology and Toxicology, Wilhelmstr. 56 72074 Tuebingen
Prof. Dr. Dr. Bernd Nürnberg
Prof. Dr. Sandra Beer-Hammer and PhD students
Type of Research Project
- Basic science
What is the background of the project?
Our working group focuses on the adaptor protein family SLy. Over the last 15 years we have demonstrated a role of SLy1 for the development of the immune system and the regulation of immune responses towards infection and tumors. In parallel we have demonstrated a role for SLy2 for the defense against Pneumococci infection in Down syndrome patients. For both SLy proteins it is still not fully understood how they act in diverse signaling pathways. This we aim to investigate with the help of SLy-deficient and/or –transgenic mouse models using in vitro, ex vivo and in vivo methods. Aim of the project: Role of the adaptor proteins SLy1 and SLy2 for the immune system and the development of immune system regulated diseases. Techniques and methods: SLy1- and SLy2-defient mouse lines, Fluorescence-activated cell sorting (FACS-Analysis), cell culture, Enzyme-linked Immunosorbent Assay (ELISA), Polymerase chain reaction (PCR), Western Blot (protein immunoblot)
What is the aim of the project?
Role of the adaptor proteins SLy1 and SLy2 for the immune system and the development of immune system regulated diseases
What techniques and methods are used?
SLy1- and SLy2-defient mouse lines, Fluorescence-activated cell sorting (FACS- Analysis), cell culture, Enzyme-linked Immunosorbent Assay (ELISA), Polymerase chain reaction (PCR), Western Blot (protein immunoblot)
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
The student(s) will be trained by PhD students to analyze mouse lymphocytes through Fluorescence-activated cell sorting (FACS), Polymerase chain reaction (PCR) or immunoblots. The student will learn how these methods work and how to perform them. If the student is experienced he/she has the chance to get and perform their own project. The student then has to present his/her results in the progress report seminar.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Texts for preliminary reading will be provided; seminars can be attended during the exchange.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster - The student will prepare a presentation
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
General practical experience in the lab (e.g. calculating concentrations, pipetting upon a scheme,...) Previous experience with: Pipetting, calculation of concentrations
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Students in biomedical fields
- Beer et al.; Molecular cloning and characterization of a novel S3 protein (SLY) preferentially expressed in lymphoid cells 2001
- Astoul et al.; Approaches to define antigen receptor-induced serine kinase signal transduction pathways 2003
- Beer et al.; Impaired immune responses and prolonged allograft survival in Sly1 mutant mice 2005
- Scheikl et al.; Reduced notch activity is associated with an impaired marginal zone B cell development and function in Sly1 mutant mice 2009
- Reis et al.; Reduced notch activity is associated with an impaired marginal zone B cell development and function in Sly1 mutant mice 2009
- Schäll et al.; SLy1 regulates T-cell proliferation during Listeria monocytogenes infection in a Foxo1-dependent manner 2015
- Arefanian et al.; Deficiency of the adaptor protein SLy1 results in a natural killer cell ribosomopathy affecting tumor clearance 2016
- von Holleben et al.; Immunoinhibitory adapter protein Src homology domain 3 lymphocyte protein 2 (SLy2) regulates actin dynamics and B cell spreading 2011
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