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Three-dimensional ultrastructural analysis of various cells, tissues, and organs by FIB/SEM tomography
Japan (IFMSA-Japan) - Kurume University, Kurume
Department of Anatomy
Kei-ichiro Nakamura, M.D., Ph.D
Kei-ichiro Nakamura, M.D., Ph.D and other lab members
Type of Research Project
- Basic science
What is the background of the project?
In the field of histology, our laboratory has done three-dimensional ultrastructural analysis with FIB/SEM (focused ion beam / scanning electron microscope) to understand the ingenious strategies of living things. This method was reported for the first time in 2008, and our laboratory adopted it in April 2011 as one of the pioneers of Japan. We are addressing around 20 research themes simultaneously with the instrument at present. Some examples are ”the develoment and enhancement for the morphological observation methods”, “the morphological analyses of relationships between organelles in connection with pathophysiology”, ”Intercellular relationships of fibroblasts or interstitial cells in various tissues and organs”. The participants can choose one with his/her interest.
What is the aim of the project?
The aim of this project is to know the three dimensional fine morphology of biological structures that are not well understood so far, such as cellular network formation in mesenchymal tissues, by the method called FIB/SEM tomography.
What techniques and methods are used?
Three-dimensional ultrastructural analysis with FIB/SEM (focused ion beam/ scanning electron microscope) to reveal functions of biological structures. The FIB/SEM is a new type of electron microscope in which you can obtain more than 1,000 serial images of cells or tissues. You analyse some intereted structure/structures within the stack of serial images on a computer image analysis software and reconstruct three dimenstional structure of them (FIB/SEM tomography). You can reveal biological nano-structures that have not even imagined.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
The students participate in 1) sample preparation, 2) observation and bio imaging on the novel electron microscope, and also 3) image analysis on PC image analysis softwares in order to find out the wonder of the nature, finally relishing research activities. The participants will achieve practical skills and ideas how to produce tissue preparations for electron microscopic observations, imaging of living cells, and data analysis of biological images to analyse by computer system, on which they will obtain a very basic way of scientific thinking and research capacity. If they get good results during the restricted period, we are welcome to help them to present the results in a scientific meeting or publish short paper in some journals.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster - The student’s name will be mentioned in a future publication - The student will have the opportunity to present the results together with the supervisor at a conference
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Basic knowledge of anatomy and histology.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Students in biomedical fields
- 1.Hashitani Hikaru; Michael Nguyen; Haruka Noda; Retsu Mitsui; Ryuhei Higashi; Keisuke Ohta; Kei-Ichiro Nakamura; Richard Lang. Interstitial cell modulation of pyeloureteric peristalsis in the mouse renal pelvis examined using FIBSEM tomography and calcium indicators. Pflügers Archiv - European Journal of Physiology (2016) DOI: 10.1007/s00424-016-1930-6
- 2.Hirashima Shingo; Keisuke Ohta; Tomonoshin Kanazawa; Satoko Okayama; Akinobu Togo; Naohisa Uchimura; Jingo Kusukawa; Kei-ichiro Nakamura. Three-dimensional ultrastructural analysis of cells in the periodontal ligament using focused ion beam/scanning electron microscope tomography. Scientific Reports 6; 39435 (2016) doi:10.1038/srep39435
- 3.Yoshitomi Munetake; Keisuke Ohta; Tomonoshin Kanazawa; Akinobu Togo; Shingo Hirashima; Kei-ichiro Uemura; Satoko Okayama; Motohiro Morioka; Kei-ichiro Nakamura. Three-dimensional ultrastructural analyses of anterior pituitary gland expose spatial relationships between endocrine cell secretory granule localization and capillary distribution. Scientific Reports 36019 (2016) doi: 10.1038/srep36019
- 4.Kanazawa Tomonoshin; Masafumi Gotoh; Keisuke Ohta; Hirokazu Honda; Hiroki Ohzono;HisaoShimokobe; Naoto Shiba; and Kei-ichiro Nakamura. Histomorphometric and ultrastructural analysis of the tendon-bone interface after rotator cuff repair in a rat model. Scientific Reports (2016) 33800 doi: 10.1038/srep33800
- 5.Kitahara Yosuke; Keisuke Ohta; Hiroshi Hasuo; Takahide Shuto; Mahomi Kuroiwa; Naoki Sotogaku; Akinobu Togo; Kei-Ichiro Nakamura; Akinori Nishi. Chronic Fluoxetine Induces the Enlargement of Perforant Path-Granule Cell Synapses in the Mouse Dentate Gyrus. PLoS ONE Jan 20 (2016) DOI:10.1371/journal.pone.0147307
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