Three-dimensional ultrastructural analysis of cells, tissues, and subcellular structures by FIB/SEM tomography
Japan (IFMSA-Japan) - Kurume University, Kurume
Department of Anatomy and Developmental Anatomy
Kei-ichiro Nakamura, M.D.
Kei-ichiro Nakamura, M.D., Ph.D and other lab members
English, Japanese
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No Yes No No Yes Yes Yes No
Type of Research Project
- Basic science
What is the background of the project?
In the field of histology and cell biology, our laboratory has done three-dimensional (3D) ultrastructural analysis with FIB/SEM (Focused ion beam / Scanning electron microscope) to reveal the functions. This method was reported for the first time in 2008, and our laboratory adopted it in as one of the pioneers. We are addressing around 20 research themes simultaneously with the instruments at present. ”The precise 3D structures of various cells”, “the relationship between organelles and pathology”, ”inter-celullar relations in constructing tissues”
What is the aim of the project?
The aim of the project is to know the 3D fine construction of biological structures, such as inter-celullar network of mensenchymal tissue, by FIB/SEM tomography and to understand the ingenious structural strategies of living things. The students participate in 1) sample preparation, 2) observation and bio imaging on the novel electron microscope, and also 3) image analysis on PC image analysis softwares in order to find out the wonder of the nature, finally relishing research activities.
What techniques and methods are used?
Various kinds of light microscopy, confocal and multi laser microscopy, and electron microscopy including 3D ultrastructural analysis with FIB/SEM (focused ion beam / scanning electron microscope) and CLEM (correlative light and electron microscopy).
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
If the student(s) want to learn anatomy, histology, and cell biology, we are ready to help them as one of the specialists of the fields. We hope the student(s) to deeply understand about morphology of human beings and animals, and acquire morphological observation techniques, such as various kinds of light microscopy and electron microscopy, including FIB/SEM tomography to reveal three dimensional structures of cells and tissues, finally love to understand the structure of living things.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
We, whole staffs of our department, will provide some lectures and demonstration courses in order to understand the theory of light and electron microscopy and know how to utilize them and also read and analyze the result images, including AI (artificial intelligence) assisted image analysis.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student’s name will be mentioned in a future publication
- The student will have the opportunity to present the results together with the supervisor at a conference
- The student will prepare an abstract
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Subjects passed: Basic knowledge of anatomy and histology
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- Histological study of costal cartilage after transplantation and reasons for avoidance of postoperative resorption and retention of cartilage structure in rats. Rikimaru-Nishi Yukiko; Hideaki Rikimaru; Shinichiro Hashiguchi; Tomonoshin Kanazawa; Keisuke Ohta; Kei-Ichiro Nakamura; Kensuke Kiyokawa. J Plastic Surg Hand Surg 52(6) 2018 352-358 | Received 21 Feb 2018; Accepted 20 Jul 2018; Published online: 04 Oct 2018
- Cytomatrix proteins CAST and ELKS regulate retinal photoreceptor development and maintenance. Hagiwara Akari; Yosuke Kitahara; Chad Paul Grabner; Christian Vogl; Manabu Abe; Ryo Kitta; Keisuke Ohta; Keiichiro Nakamura; Kenji Sakimura; Tobias Moser; Akinori Nishi; Toshihisa Ohtsuka. J. Cell Biol. 2018 Vol. 217 No. 11 3993–4006 Published Online: 6 September; 2018 | Supp Info:
- Uncoupled mitochondria quickly shorten along their long axis to form indented spheroids; instead of rings; in a fission-independent manner. Miyazono Yoshihiro; Shingo Hirashima; Naotada Ishihara; Jingo Kusukawa; Kei-ichiro Nakamura; and Keisuke Ohta. Sci Rep 8:350; 2017 | DOI:10.1038/s41598-017-18582-6
- Role of mucosa in generating spontaneous activity in the guinea pig seminal vesicle. Takeya Mitsue; Hikaru Hashitani; Tokumasa Hayashi; Ryuhei Higashi; Kei-ichiro Nakamura; Makoto Takano. J Physiol 595.14 (2017) pp 4803–4821 DOI: 10.1113/JP273872
- Three-dimensional analysis of somatic mitochondrial dynamics in fission-deficient injured motor neurons using FIB/SEM. TAMADA HIROMI; SUMIKO KIRYU-SEO; Hiroki Hosokawa; KEISUKE OHTA; Naotada Ishihara; Masatoshi Nomura; Katsuyoshi Mihara; KEIICHIRO NAKAMURA; Hiroshi Kiyama. J Comp Neurol; 2017 DOI:10.1002/cne.24213