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Three-dimensional ultrastructural analysis of cells, tissues, and subcellular structures by FIB/SEM tomography
Japan (IFMSA-Japan) - Kurume University, Kurume
Department of Anatomy and Developmental Anatomy
Kei-ichiro Nakamura, M.D.
Kei-ichiro Nakamura, M.D., Ph.D and other lab members
Type of Research Project
- Basic science
What is the background of the project?
In the field of histology and cell biology, our laboratory has done three-dimensional (3D) ultrastructural analysis with FIB/SEM (Focused ion beam / Scanning electron microscope) to reveal the functions. This method was reported for the first time in 2008, and our laboratory adopted it in as one of the pioneers. We are addressing around 20 research themes simultaneously with the instruments at present. ”The precise 3D structures of various cells”, “the relationship between organelles and pathology”, ”inter-celullar relations in constructing tissues”
What is the aim of the project?
The aim of the project is to know the 3D fine construction of biological structures, such as inter-celullar network of mensenchymal tissue, by FIB/SEM tomography and to understand the ingenious structural strategies of living things. The students participate in 1) sample preparation, 2) observation and bio imaging on the novel electron microscope, and also 3) image analysis on PC image analysis softwares in order to find out the wonder of the nature, finally relishing research activities.
What techniques and methods are used?
Various kinds of light microscopy, confocal and multi laser microscopy, and electron microscopy including 3D ultrastructural analysis with FIB/SEM (focused ion beam / scanning electron microscope) and CLEM (correlative light and electron microscopy).
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
If the student(s) want to learn anatomy, histology, and cell biology, we are ready to help them as one of the specialists of the fields. We hope the student(s) to deeply understand about morphology of human beings and animals, and acquire morphological observation techniques, such as various kinds of light microscopy and electron microscopy, including FIB/SEM tomography to reveal three dimensional structures of cells and tissues, finally love to understand the structure of living things.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
We, whole staffs of our department, will provide some lectures and demonstration courses in order to understand the theory of light and electron microscopy and know how to utilize them and also read and analyze the result images, including AI (artificial intelligence) assisted image analysis.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student’s name will be mentioned in a future publication - The student will have the opportunity to present the results together with the supervisor at a conference - The student will prepare an abstract
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Subjects passed: Basic knowledge of anatomy and histology
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
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