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Piperine-free Piper nigrum extract: a potent breast cancer preventive supplement
Thailand (IFMSA-Thailand) - Faculty of Medicine, Songkla University, Songkhla
Department of Biomedical Sciences
Assoc. Prof. Dr. Surasak Sangkhathat
Assoc. Prof. Dr. Potchanapond Graidist
Type of Research Project
- Basic science
What is the background of the project?
In our previous studies, Piperine-free Piper nigrum extracts (PFPE) demonstrated potent cytotoxic effects on breast cancer cell lines (MCF-7 and MDA-MB-468) by mediating the induction of the apoptotic pathway. In addition, the findings of lower cytotoxicity and slower growth rate of tumor incidence following a single oral administration of PFPE in female Sprague-Dawley rats with NMU-induced mammary tumors led to the concept that the PFPE might play a role in breast cancer prevention. Although, the mechanism of action of its constituents as a cancer preventive agent needs to be further investigated. Presently, the conventional method using organic solvents is used to extract PFPE. Most of the organic solvents are flammable, volatile and often toxic to the environment. They can contribute to environmental pollution and the greenhouse effect. Therefore, our team would also explore the extraction methods of PFPE in order to develop a safer way and increase the extraction yield.
What is the aim of the project?
1. To investigate the mechanisms of PFPE as a dietary supplement in breast cancer prevention. 2. To develop a simple, high throughput, safe, and environmental friendly approach using different extraction methods of piperine-free Piper nigrum (PFPE)
What techniques and methods are used?
1. PFPE extraction procedure 1.1 Conventional extraction 2. Qualification of PFPE 2.1 Liquid chromatography – Mass Spectromeer (LC-MS) based identification of active pure compounds concentration in PFPE 2.2 Structures confirmation of PFPE by Nuclear Magnatic Resonance (NMR) 2.3 Formulation optimization of PFPE 3. In vitro study (cytotoxicity study) 3.1 Cell lines and culture conditions 3.2 Cytotoxicity test (MTT assay) 3.3 Western blot analysis 4. In vivo study (Animal model) 4.1 Acute toxicity study (mice) 4.2 Cancer preventive activity study (rat)
What is the role of the student?
- The student will mainly observe
What are the tasks expected to be accomplished by the student?
The student will be able to perform cytotoxicity study (in vitro) like cell culture, MTT assays, and Western blot analysis. We also conduct in vivo studies in rat animal models. The student can observe and participate, if they wish to, in the cancer preventive activity studies in rats. All tasks will be supervised by our team. The student is expected to discuss his/her overall understandings of the project with the team.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster - The student will prepare a presentation
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
The student must have basic knowledge in Life Science and Biomedical Science.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields - Dental medicine students (IADS members)
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