Impact of major histocompatibility complex (MHC) molecule stimulation on gene expression in lymphocytes
Germany (bvmd) - Martin-Luther-Universitaet Halle-Wittenberg, Halle a.d.Saale
Department of Pediatrics I, Martin Luther University Halle-Wittenberg, Ernst-Grube-Str. 40, 06120 Halle
Prof. Dr. Jan-Henning Klusmann
Prof. Dr. Martin S. Staege
English, German
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No Yes Yes Yes No No Yes Yes Yes No
Type of Research Project
- Basic science
What is the background of the project?
T cells recognize peptides in combination with major histocompatibility complex (MHC) molecules on antigen presenting cells (APC). The interaction between MHC molecules and the T cell receptor (TcR) delivers an signal to the T cell. It seems possible that the engagement of the MHC molecule by the TcR can also deliver a signal to the APC. Such signals might be relevant for instance in the context of reciprocal antigen presentation between T cells. It is known that this special form of antigen presentation can lead to an unusual T cell activation which can result in so-called bystander lysis of target cells T cells without necessity for antigen presentation by these bystander cells. This form of T cell activation might have implications for virus infections and cancer.
What is the aim of the project?
The project aims in the analysis of the effects of stimulation of MHC molecules on lymphocytes in vitro.
What techniques and methods are used?
Primary human lymphocytes will be isolated from blood from healthy donors. MHC stimulation will be performed with immobilized antibodies. Gene expression of cells after stimulation will be analyzed with quantitative reverse transcription-polymerase chain reaction and flow cytometry (and - depending on the results - microarrays).
What is the role of the student?
What are the tasks expected to be accomplished by the student?
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
No special skills are required
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- Staege et al.; Cytotoxic T cells with reciprocal antigenic peptide presentation function are not generally resistant to mutual lysis. Immunol Cell Biol. 2003;81:266-74
- Staege et al.; Consequences of antigen self-presentation by tumor-specific cytotoxic T cells. Immunobiology. 2000;201:332-4
- Staege et al.; The antigen self-presentation function of the cytotoxic T-cell clone 10BK.1 depends on reciprocal peptide presentation. Immunology. 1994;81:333-7
- Staege et al.; DNA-microarrays as tools for the identification of tumor specific gene expression profiles: applications in tumor biology; diagnosis and therapy. Klin Padiatr. 2003;215:135-9