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Role of Cancer-associated Fibroblasts on Cholangiocarcinoma Progression
Thailand (IFMSA-Thailand) - Faculty of Medicine, Khon Kaen University, Khon Kaen
Assoc. Prof. Rasana Wongratanacheewin
Assist. Prof. Kulthida Vaeteewoottacharn, Ms. Paweena Dana, Ms. Paksiree Saranaruk
Type of Research Project
- Basic science
What is the background of the project?
It is well-known that the interaction between tumor microenvironment and the tumor itself is significant in multiple steps of cancer development, progression, and metastasis. In cholangiocarcinoma (CCA), a common disease in the North-Eastern region of Thailand, the unique presentations of abundant desmoplastic stromal reaction or high content of intra-tumoral and juxta-tumoral fibrosis are evident. The role of cancer-associated fibroblasts (CAF) is now being studied regarding its role in the treatment of CCA. However, little is know about the cellular interaction between CAF and CCA both in vitro and in vivo. The candidate molecules that were produced by or secreted from CCA cell and interact with CAF to promote CCA growth and metastasis will be determined. Ultimately, the biomarkers identified from this study might be used as novel targets for improving CCA treatment. This study aims to explore the functional significance of CAF on CCA growth and metastasis as well as to determine the candidate molecules in CAF-CCA interaction. Techniques used are mammalian cell culture technique, cell proliferation assay, cell migration assay by wound scratching technique, Western blot, immunohistochemistry, and CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats).
What is the aim of the project?
1. Determination of functional significance of CAF on CCA growth and metastasis in vitro and in vivo 2. Identification the candidate molecules that functions in CAF-CCA interaction in the in vitro cultured condition as well as in the CCA tissues 3. Determination of the roles of candidate molecules by interfering the expressions or function
What techniques and methods are used?
1. Mammalian cell culture technique 2. Cell proliferation assay 3. Cell migration assay by wound scratching technique 4. Western blot 5. Immunohistochemistry 6. RNA interference or CRISPR (Clustered regularly interspaced short palindromic repeats) technique
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
The student will have the opportunity to work with the researcher. The cell culture related technique as well as the immunohistochemistry technique using CCA tissues are frequently performed here. If he/she is interested in those two techniques, he/she will be able to perform the experiment, interpret the result, and perform the analysis under our supervision.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster - The student’s name will be mentioned in a future publication
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
The preliminary readings are requested for all techniques prior to the experiment observation and participation.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields - Dental medicine students (IADS members)
- Vaeteewoottacharn K; et al. Inhibition of carbonic anhydrase potentiates bevacizumab treatment in cholangiocarcinoma. Tumour Biol. 2016 Jul; 37(7):9023-35.
- Vaeteewoottacharn K; et al. Potential targeted therapy for liver fluke associated cholangiocarcinoma. J Hepatobiliary Pancreat Sci. 2014 Jun; 21(6):362-70.
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