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Prevalence of β-lactam Aminoglycoside antibiotics resistance genes among bacterial pathogenic isolates in Egypt. (Misr University)
Egypt (IFMSA-Egypt) - Misr University for Science and Technology, 6th of October
Research department, College of Biotechnology, MUST University, Giza, Egypt.
Prof. Dr. Mokhtar El Zawahry.
Prof. Dr. Mohamed Mostafa.
Type of Research Project
- Basic science
What is the background of the project?
The misuse of antibiotics led to serious problems one of these problems is the development of multidrug resistant bacteria (MDR) ( Daoud et al.,2015).The prevalence of MDR bacteria results in high rates of morbidity and mortality worldwide(Huang et al.,2016). These types of bacteria could have several resistance mechanisms might be single or multiple(Bagheri-Nesami et al.,2016). One of these types extended-spectrum beta-lactamases (ESBLs) are resistance enzymes that stop the work of penicillins, cephalosporins & other classes of antibiotics because plasmids that encoding the gene of ESBLs, almostly carry other resistant indications (Warjri et al.,2015 & Fróes et al.,2016). However, current knowledge data describe the development of multidrug resistant genes that could be acquired through different genetic transfer mechanisms in
What is the aim of the project?
Therefore, the current study aimed to determine the most prevalent genes responsible for resistance against β-lactam and Aminoglycoside antibiotics among Egyptian clinical bacterial isolates.
What techniques and methods are used?
• DNA extraction from bacteria. • Polymirase Chain Reaction "PCR" technique. • Agarose Gel electrophoresis. • Visualization and documentation of gel electrophoresis.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
• Isolation of bacterial genomic DNA. • Amplification of specific primers using real time PCR and sequencing of PCR products. • Visualization and documentation of PCR product(s) via agarose gel electrophoresis. • Antibiotic susceptability tests on isolates from agarose gel electrophoresis. • Analysis of data (including standard curve quantification, comparetive quantification and normalization of results; statistical analysis and visualization).
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Theoretical teaching about the basics of molecular biology and PCR techniques
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster - The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Pre-Medical students from the American-British system
- Inactivation of antibiotics and the dissemination of resistance genes. Davies J. Science. 1994 Apr 15;264(5157):375-82. Review.
- The prevalence of aminoglycoside resistance and corresponding resistance genes in clinical isolates of staphylococci from 19 European hospitals. Franz-Josef Schmitz. Ad C. Fluit. Mechthild Gondolf. Ralf Beyrau. Elke Lindenlauf. Jan Verhoef. Hans-Peter Heinz. Mark E. Jones. J Antimicrob Chemother (1999) 43 (2): 253-259.
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