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Implantation of neural precursors, derived from human induced pluripotent stem cells, into the brain of the nude rat after experimental traumatic brain injury
Germany (bvmd) - Universitaet zu Koeln, Cologne
Institute of Neurophysiology, Robert Koch Str. 39, 50931 Köln
Prof. Dr. Jürgen Hescheler
Marek Molcanyi MD, PhD, Firuze Fulya Hatay MSc.
Type of Research Project
- Clinical Project without Laboratory work
What is the background of the project?
Neural precursors, derived from human induced pluripotent stem (iPS) cells, will be implanted into the brains of healthy nude rats and into the brains subjected to an experimental traumatic brain injury (fluid-percussion-model) reflecting much of the pathophysiology of the clinically relevant human closed head trauma. Nude rats are necessary, as we are testing the potential of human-iPS-cell derived precursors. Induced pluripotent stem cells are a type of pluripotent stem cell that can be generated directly from adult cells. Pluripotent stem cells hold great promise in the field of regenerative medicine. Because they can propagate indefinitely, as well as give rise to every other cell type in the body, they represent a single source of cells that could be used to replace those lost to damage or disease.
What is the aim of the project?
What is the aim of the project? Exploring the restorative/regenerative potential of iPS-derived neural precursors in a clinically relevant experimental set-up.
What techniques and methods are used?
What techniques and methods are used? First of all we will use molecular-biological techniques including genetic manipulation and cell culturing. After induction of experimental traumatic brain injury, we will use stereotactic implantation of the stem cells into the brain and in-vivo imaging as well as transcardial perfusion and sectioning. At least we will use histological/immunohistochemical evaluation of the process.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
The student will participate in cell culture techniques including genetic manipulation as well as cell culturing under close supervision. Animal experiments and operations on animals will be carried out by trained microsurgeons. The student will have the change to observe these operations and participate in animal experiments under close supervision. Tissue processing and laboratory histological/immunohistochemical evaluation will be done by the student partly independent.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Introductory lecture and advanced reading will be provided
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation - The student will prepare an abstract - The student’s name will be mentioned in a future publication
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
What skills are required of the student? all required skills will be trained along the way of the experimental work Is there any special knowledge or a certain level of studies needed? x Subjects passed: scientific proficiency in English language x Previous experience with: reading/understanding/interpreting scientific manuscripts
Are there any legal limitations in the student’s involvement
Operations on animals may be carried out only by trained and certified microsurgeons (min. requirement FELASA B), additionally the student must have an insurance for potential damage to the lab. equipment and possible injuries/contamination during the lab/experimental work.
Type of students accepted
This project accepts: - Medical students - Pre-Medical students from the American-British system - Students in biomedical fields - Dental medicine students (IADS members)
- Reversal of neuromotor and cognitive dysfunction in an enriched environment combined with multimodal early onset stimulation after traumatic brain injury in rats - Maegele M1; Lippert-Gruener M; Ester-Bode T; Sauerland S; Schäfer U; Molcanyi M; Lefering R; Bouillon B; Neiss WF; Angelov DN; Klug N; McIntosh TK; Neugebauer EA
- pubmed record of Marek Molcanyi
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