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Immunohistochemical expression of Desmocollin2 and CK14 in urothelial carcinoma. (Menoufia University)
Egypt (IFMSA-Egypt) - Menoufia University, Menoufia
Pathology department faculty of medicine Menofia University.
Prof Dr/ Hayam Aiad
Dr. Hala said. - Dr. Shereen fathy - Dr.Lamia Sabry
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Urothelial carcinoma (UC) includes the majority of bladder cancers. However, UC has a propensity for divergent differentiation. UC with squamous differentiation, which is the most common mixed histological feature, occurs in 10–60% of UC cases. The squamous carcinoma has worse prognosis than pure Urothelial carcinoma “UC” and it might be an indicator of poor response to chemotherapy and radiotherapy. Therefore, it is important to detect the existence of squamous differentiation in bladder cancer.
What is the aim of the project?
Evaluate diagnostic value of. Desmocollin 2 and CK14 for squamous differentiation in urothelial carcinoma as well as correlation of both marker expression with clinic pathologic parameters of tumor as tumor grade and stage, Urothelial carcinoma (UC) with squamous differentiation tends to present at higher stages than pure UC. To distinguish UC with squamous differentiation from pure UC, a sensitive and specific marker is needed. Desmocollin 2 (DSC2) is a protein localized in desmosomal junctions of stratified epithelium, but little is known about its biological significance in bladder cancer. We examined the utility of DSC2 as a diagnostic marker.
What techniques and methods are used?
Tissue Samples: - Primary tumor samples were collected from Menofia university teaching hospital patients with bladder cancer .Patients were treated at the department. For immunohistochemically analysis, we used archival formalin-fixed, paraffin- embedded tissues from these patients, who had undergone radical cystectomy for bladder cancer, because radical cystectomy can give precise information on pathological stage and histology. Hematoxylin and eosin- stained sections from each case were re-evaluated histopathologically by two investigators Dr.Hala said And Dr.shereen fathy. The cancer cases were histologically classified as pure UC and squamous differentiation is around 60% of the UC cases. Immunohistochemistry: - (IHC) refers to the process of selectively imaging antigens (e.g. proteins) in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues. The aim is to differentiate and detect the squamous carcinoma type in the UC by detecting the Desmocollin2 and CK14. Statistical Methods: - Making statistics about the antigens or markers “CK24 and desmocillin2” of the squamous differentiation in urothelial carcinoma and the survival rates of their patients or the death rates to determine the final practical prognosis.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
1- The student will assist in the whole methods of the research which includes the tissue sampling, the immunohistochemistry and the statistical methods. 2- The student will have to attend at the pathology department for six hours for four days each week. 3- The student will write down all the above mentioned steps in his/her logbook to be signed by the tutors at the end of each shift. 4- At the end of the clerkship the student will make the conclusion of it according to the statistical studies they already made through the whole month to evaluate the squamous differentiation in the urothelial carcinoma.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report - The student’s name will be mentioned in a future publication
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
basic knowledge in research , communication skills
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system
- Hayashi; T.; Sentani; K.; Oue; N.; Anami; K.; Sakamoto; N.; Ohara; S.; Teishima; J.; Noguchi; T.; Nakayama; H.; Taniyama; K.; Matsubara; A. and Yasui; W. (2011); Desmocollin 2 is a new immunohistochemical marker indicative of squamous differentiation in urothelial carcinoma. Histopathology; 59: 710-721.
- Wenbin Huang; Sean R. Williamson; Qiu Rao; Antonio Lopez-Beltran; Rodolfo Montironi; John N. Eble; David J. Grignon; Muhammad T. Idrees; Robert E. Emerson; Xiao-jun Zhou; Shaobo Zhang; Lee Ann Baldridge; Noah M. Hahn; Mingsheng Wang; Michael O. Koch; Liang Cheng; Novel markers of squamous differentiation in the urinary bladder; Human Pathology; Volume 44; Issue 10; 2013; Pages 1989-1997
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