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[Tunis]Diagnosis of bacterial infections from specimen treatment to bacterial identification and susceptibility testing.ect
Tunisia (ASSOCIA-MED) - University of Tunis El-Manar/Faculty of Medecine of Tunis, Tunis
Laboratory of bacteriology and virology , Charles Nicolle Hospital , Tunis – Tunisia
Pr. Slim Amine
Pr. Boutiba Ilhem / Dr. Saidani Mabrouka / Dr. Kanzari Lamia / Dr Ferjani Asma
Type of Research Project
- Basic science
What is the background of the project?
The bacteriological diagnosis of serious bacterial infections is essential. It depended on specimen quality; correct bacterial identification of the pathogens involved and on the study of their susceptibility to antibiotics. This will allow clinicians to adjust their first-line antibiotics; which is itself guided by the annual bacterial susceptibility records of main bacteria isolated in our hospital. Also, within the framework of the research, detection of the main genes coding the resistance to beta-lactams using PCRs (polymerase chain reaction) and sequencing will be useful.
What is the aim of the project?
The aim of the project is to practice the different stages of the diagnosis of the main bacterial infections caused by non-fastidious bacteria and to study their susceptibility to antibiotics.
What techniques and methods are used?
-Specimen analysis (microscopic steps and culture) and result interpretation -Bacterial identification (Gram staining, Api system) -Susceptibility testing (diffusion method, determination of Minimal -Inhibitory Concentration (MIC)) -PCR and sequencing
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The student will mainly observe
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
What are the tasks expected to be accomplished by the student?
Tasks expected to be accomplished by the student: For the main specimen reserved (blood, urine, several fluids, stool and pus) 1-Specimen analysis (microscopic steps and culture) and result interpretation 2- Bacterial identification (Gram staining, Api system) 3- Susceptibility testing (diffusion method, determination of Minimal Inhibitory Concentration (MIC)) Tasks expected to be done under supervision: PCR and sequencing
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes there will be
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Basic knowledge about: 1- Main bacteria causing serious human infections, including nosocomial 2-infections 3-Antibiotics (classification, mechanisms of action and resistance)
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months)
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