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Influence of murine cytomegalovirus on BV-2 microglial cells
Croatia (CroMSIC) - University of Rijeka, Rijeka
Department of Physiology and Immunology
Pero Lučin PhD, MD
Valentino Rački, MD
Type of Research Project
- Basic science
What is the background of the project?
Cytomegalovirus presents one of the challenges we face in modern microbiology, especially in patients who are immunocompromised (transplanted patients, HIV). For that reason, it is important to understand the effects it has on our body and cells. Our research is focused on finding the effects of cytomegalovirus on microglial cells and whether it can modulate the cells to become suppressed or proinflammatory. Microglial cells are the first responders in the brain, which is one of the reasons why we chose to focus on them. It presents the opportunity to study one of the first cells that respond to infection. These cells have been a part of interesting crosstalks regarding CMV infection, cognitive dysfunction and even tumor development.
What is the aim of the project?
Aim of the project is to study the effects of murine cytomegalovirus on BV-2 microglial cells.
What techniques and methods are used?
We use immunofluorescence (a technique used fot light microscopy and primarily on biological samples, in this case on microglial cells), Western blot (an analytical technique used to detect proteins in tissue, in this case in brain tissue), flow cytometry (a laser or impedance-based biophysical technology employed in cell counting, cell sorting, biomarker detection and protein engineering, by suspending cells in a stream of fluid and passing them by an electronic detection apparatus) and real-time metabolic assessments (Seahorse) in our research.
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Lab work and performing various methods will be expected of the student. The work will be done under supervision, unless it is deemed the student is proficient enough. Furthermore, the student will be provided with materials and is expected to read up on some theory before coming on exchange. As mentioned previously, the methods include immunofluorescent, Western blot, flow cytometry and Seahorse, though we can’t guarantee which will be used during exchange periods. We will communicate that to the student prior to arrival.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Theoretical teaching will be provided by the tutor, along with a weekly journal club which will cover a topic.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will have the opportunity to present the results together with the supervisor at a conference
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Subjects passed: microbiology, neurophysiology, physiology Previous experience with: Basic lab work and techniques (theory)
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months)
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