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Molecular alterations in hematologic myeloid pathology
Spain (IFMSA-SPAIN) - University of Las Palmas de Gran Canaria, Las Palmas
Teresa Molero Labarta
Cristina Bilbao Sieyro
Type of Research Project
- Basic science
What is the background of the project?
This study consists in the determination of genetic alterations in myeloid pathology, as well as the genome expression levels in these patients. To be more concrete, we focus on myeloproliferative syndromes and acute myeloid leukemia. Because of the variability of the clinical expression, we also try to find other clinical values to detect this pathology because no genetic alterations are found in many cases. These refer to a group of diseases of the bone marrow in which excess cells are produced. Acute myeloid leukemia (AML) is a cancer of the myeloid line of blood cells, characterized by the rapid growth of abnormal white blood cells that build up in the bone marrow and interfere with the production of normal blood cells. AML is the most common acute leukemia affecting adults, and its incidence increases with age. Although AML is a relatively rare disease, its incidence is expected to increase as the population ages. As an acute leukemia, AML progresses rapidly and is typically fatal within weeks or months if left untreated.
What is the aim of the project?
The aim is to find molecular markers that help in a better characterization of hematological myeloid pathologies; expecting it to have value in the prognostic, diagnosis, as a marker of minimal residual disease or even for analyze treatment respond. a) alteration in expression levels of epigenetic modulators BRD4, EZH2 and ASXL1 being BRD4 a novel marker and a potential therapeutic target b) correlation mRNA and protein levels c) binding of BRD4 to genomic targets through chromatin immunoprecipitation techniques d) determine whether expression levels could offer new insight as prognostic and minimal residual disease markers e) associations among this variables and molecular markers routinely analyze in our laboratory f) association with clinical variables and progression.
What techniques and methods are used?
Polymerase Chain Reaction (PCR) detection of antigens, Sanger DNA sequencing, Massive DNA parallel sequencing, Electrophoresis.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
At first time, the student will read literature about the clinical pathology to get a better understanding of what kind of clinical values we search for. It is also needed to learn the bases of the techniques that will be used, and then the student will observe how their tutors do it. After, when the student has understood it, he/she could prepare polymerase chain reaction related techniques independtly, and therefore, analise the generated data with the help of the tutor. Tutors will supervise him/her work.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes, the project will be deeperly introduced upon arrival. In futher days, a brief theorical class before the approach to the experimients and some bibliography will be provided.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Students only need to have basic science knowledge.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system - Students in biomedical fields - Dental medicine students (IADS members)
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