Projects
Name
Galectin-3 alters the adaptative immune response in Spinal Cord Injury in mice model and promote axonal regeneration
University
Medicine School Souza Marques
Domain
Neurology
Departement
Laboratory of Neural degeneration and Repair
Head
Prof. Ana Maria Blanco Martinez MD PhD
Tutor
Caio Andrade Prins Rodrigues MD
Languages
English, Portuguese
Duration
8 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
Yes Yes Yes No No No No No No Yes Yes Yes
Type of Research Project
- Basic science
What is the background of the project?
We are investigating the mechanisms behind neural degeneration after a spinal cord injury model (contusion-concussion model with a vessel aneurysm 30g clip) and the dialog of adaptive immune response with a lectin protein called galectin- 3 in the acute and chronic processes. We will use two strands of animal: mice wild type C57BL/6, a mouse strand that normally expresses endogenous Galectin 3; C57BL/6 GAL-/- a mouse strand with a knockout for the gene LGAL that expresses Galectin-3, turning off its production. After the lesion we’re going to investigate the populational dynamics of adaptive immune cells along time and evolution, observing the functional progress and morphology changes comparing the strands cited above.
What is the aim of the project?
What is the aim of the project? To reach more comprehension about how the adaptive immune system participate in the deterioration and repair of central nervous system after a common lesion seen in society and elucidate a novel treatment to spinal cord injury. We’re also going to investigate the possibility of spinal cord injury elucidate an autoimmune reaction hampering axonal growth and a correct regeneration of the central nervous system.
What techniques and methods are used?
Used Techniques of the Project: First step: Spinal cord injury using a 9th thoracic vertebra spine level compression-contusion with a 30g vessel clip during 1 minute Second step: we will test motor function using: electoneuromyography, flow cytometry, interleukins and chemokines Enzyme Linked Immuno Sorbent Assay and Western blot, Immunohistochemistry, Light microscopy. Third step: we will analise the results with ImageJ and produce statistics about it.
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Used Techniques of the Project: First step: Spinal cord injury using a 9th thoracic vertebra spine level compression-contusion with a 30g vessel clip during 1 minute Second step: we will test motor function using: electoneuromyography, flow cytometry, interleukins and chemokines Enzyme Linked Immuno Sorbent Assay and Western blot, Immunohistochemistry, Light microscopy. Third step: we will analise the results with ImageJ and produce statistics about it.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
1
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Good english conversation and reading skills.
Are there any legal limitations in the student’s involvement
No
Hours
6
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- Pre-Medical students from the American-British system
Articles
- Prins CA; Almeida FM; Martinez AMB. Absence of galectin-3 attenuates neuroinflammation improving functional recovery after spinal cord injury. Neural Regeneration Research. 2016;11(1):92-93. doi:10.4103/1673-5374.175051.