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CD49 expression by flowcytometry and trisomy 12 detection by fluorescent in situ hybridization (FISH) in chronic lymphocytic leukemia (Assuit University)
Egypt (IFMSA-Egypt) - Assiut University, Assiut
South Egypt Oncology Institute, City Street, Assiut city, Assiut, Egypt.
Prof. Dr. Eatemad Helmy Yassin
Dr. Asmaa Ahmed Mohammed
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Chronic lymphocytic leukemia is a malignant lymphoproliferative disorder in which a disruption of normal apoptosis leads to the accumulation of immunologically incompetent mature lymphocytes. In most instances, CLL is due to the overgrowth or accumulation of immunoglobulin producing B lymphocytes. Although bone marrow examination is rarely required to make the diagnosis of CLL in general practice, it may be valuable prior to the start of the treatment in order to define prognostic factors. A number of abnormalities including trisomy 12 and presence of 14q, 11q and 17p chromosome abnormalities have been related to poor outcome. CLL is characterized by several functional interactions involving CD49d and specific chemokine-cytokine receptor/ligand. CD49d can be represented as a major factor of a complex interplay with other surface receptors, all expressed by CLL cells. There is a strong correlation between CD49d expression and trisomy 12 since it might anticipate a putative general feature of CLL cells.
What is the aim of the project?
1) The expression of CD49d in cases of CLL and its diagnostic and/or prognostic value 2) The incidence of occurrence of trisomy 12 in CLL patients and its impact on the prognostic criteria of CLL and its relation to the expression of CD49d
What techniques and methods are used?
1) Complete blood picture 2) Bone marrow aspirate 3) Bone marrow biopsy 4) Flow cytometric analysis for CD49d expression 5) Trisomy 12 detection by FISH
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
- If the project is clinical
- the student will take active part in the clinical examination
- If the project is clinical
- the student will be allowed to work with patients
- The tasks of the student will be performed on his/her own
What are the tasks expected to be accomplished by the student?
Help in the investigation of the expression of CD49d in cases of CLL and its diagnostic or prognostic value, participate in taking a complete blood picture, bone marrow biopsy help in the flow cytometric analysis for CD49d expression, and Trisomy 12 detection by FISH, also data analysis and results comprehension are expected to be done under supervision of the professor first, then done on the student’s own, and the professor will review and discuss the results with them at the end.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Lectures conecring the topic & presentation of the clinical skills used.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report - The student will prepare a poster - The student will prepare a presentation - The student will prepare an abstract
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
It's prefered if the student have studied Oncology.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
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