Projects
Name
CD49 expression by flowcytometry and trisomy 12 detection by fluorescent in situ hybridization (FISH) in chronic lymphocytic leukemia
University
Egypt (IFMSA-Egypt) - Assiut University, Assiut
Domain
Oncology
Departement
South Egypt Oncology Institute, City Street, Assiut city, Assiut, Egypt.
Head
Prof. Dr. Eatemad Helmy Yassin
Tutor
Dr. Asmaa Ahmed Mohammed
Languages
English
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No No Yes Yes Yes Yes Yes No
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Chronic lymphocytic leukemia is a malignant lymphoproliferative disorder in which a disruption of normal apoptosis leads to the accumulation of immunologically incompetent mature lymphocytes. In most instances, CLL is due to the overgrowth or accumulation of immunoglobulin producing B lymphocytes. Although bone marrow examination is rarely required to make the diagnosis of CLL in general practice, it may be valuable prior to the start of the treatment in order to define prognostic factors. A number of abnormalities including trisomy 12 and presence of 14q, 11q and 17p chromosome abnormalities have been related to poor outcome. CLL is characterized by several functional interactions involving CD49d and specific chemokine-cytokine receptor/ligand. CD49d can be represented as a major factor of a complex interplay with other surface receptors, all expressed by CLL cells. There is a strong correlation between CD49d expression and trisomy 12 since it might anticipate a putative general feature of CLL cells.
What is the aim of the project?
1) The expression of CD49d in cases of CLL and its diagnostic and/or prognostic value 2) The incidence of occurrence of trisomy 12 in CLL patients and its impact on the prognostic criteria of CLL and its relation to the expression of CD49d
What techniques and methods are used?
1) Complete blood picture 2) Bone marrow aspirate 3) Bone marrow biopsy 4) Flow cytometric analysis for CD49d expression 5) Trisomy 12 detection by FISH
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Help in the investigation of the expression of CD49d in cases of CLL and its diagnostic or prognostic value, participate in taking a complete blood picture, bone marrow biopsy help in the flow cytometric analysis for CD49d expression, and Trisomy 12 detection by FISH, also data analysis and results comprehension are expected to be done under supervision of the professor first, then done on the student’s own, and the professor will review and discuss the results with them at the end.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Lectures conecring the topic & presentation of the clinical skills used.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
It's prefered if the student have studied Oncology.
Are there any legal limitations in the student’s involvement
No
Hours
6
Type of students accepted
This project accepts:
- Medical students
Articles
- Kumar V; Abbas AK; Fausto N; Aster JC; editors. Pathological basis of disease. 8th ed. Philadelphia: Saunders; 2010.
- Amal S. Ibrahim; Hussein M. Khaled; Nabiel NH Mikhail; Hoda Baraka; Hossam Kamel. Cancer Incidence in Egypt: Res Its of the National Population-Based Cancer Registry Program. Journal o Cancer Epidemiology 2014.
- Babjuk M; Oosterlinck W; Sylvester R; Kaasinen E; Böhl A;Palou-Redorta J; et al. European Association of Urology (EAU).EAU guidelines on non- muscle- invasive urothelial carcinoma of the bladder; he 2011 update. Eur Urol 2011; 59:997-1008.
- Stacey A. Fedewa; Amr S. Soliman; Kadry Ismail; Ahmed Habias; Ibrahim A. Seifeldin; Mohamed Ramadan; Hoda G. Omar; Jerome Nriagu; and Mark L. ;Wilson: Incidence analyses of bladder cancer in the Pile delta region of (Egypt. Cancer Epidemiol. 2009 October; 33(3-4): 176—181.
- 'Wu Xifeng; Jie Lin H; Grossman Barton; Huang Maosheng; Gu Jian; Carol J; Etzel Amos Cl; Dinney CP; Spitz MR: Projecting Individualized Probabilities of Developing Bladder Cancer in White Individuals. Journal of Clinical Oncology 2007;
- Mensing IT; Speijers GJ; Meulenbelt J: Health implications of exposure to environmental nitrogenous compounds. Toxicol Rev 2003;
- Holmäng S; Hedelin H; Anderström C; Johansson Sh. The relationship among multiple recurrences; progression and prognosys of patients with stages Ta and Tl transitional cell cancer of the bladder f Ilowed for at least 20 years. J Urol 1995; 153:1823-6; discussion: 1826-7.
- Morabito F; Mangiola M; Rapezzi D; Zupo S; Oliva BM; erraris AM; et al. Expression of CDIO by B-chronic lymphocytic leukemia cells undergoing apoptosis in vivo and in vitro. Haematologica 2003; 88.864-73.
- Sumitomo M; Shen R; Walburg M; Dai J; Geng Y; Navarro D; et al. Neutrai endopeptidase inhibits proState cancer cell migration py blocking foa adhesion kinase signaling. J Clin Invest 2000; 106:1399-407