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The Role of Macrophages in Multiple Myeloma and 3D visualization in multiple myeloma bone marrow compartment analysis
Croatia (CroMSIC) - University of Rijeka, Rijeka
Department of pathology
Associate profesor Sanja Štifter
Associate profesor Sanja Štifter
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
The biopsies were analyzed for the absolute number of macrophages, the percentage of plasma cells and the ratio of their representation in the sample. Sections were evaluated using visual standard microscopic analysis and a computer assisted IHC quantification on 44 cases. All stained slides were scanned (Olympus VS110) and analyzed using Alphelys Spot Browser 2 integrated system, consisting of software controlled (Spot Browser 2.4.4, Alphelys, France) and motorized stage microscope (Eclipse 50i, Nikon, Japan) with mounted digital camera (1360x1024 resolution, 24bit, CFW1310C, Microvision, France). Overview images of the slides were taken at 20x magnification, analyzing images of the complete section area at 100x or 200x magnification, under constant light intensity, microscope and camera settings. In the further course of our study, identification and quantification of the macrophage polarization status is intended. The previously obtained image analysis results were correlated with angiogenesis as total vascular area (TVA) and clinical parameters, including Durie Salmon (DS) classification.
What is the aim of the project?
The aim of the study was to shed light more closely on the interaction between malignant plasma cells in multiple myeloma (MM) and macrophages in the bone marrow.
What techniques and methods are used?
Method: The immunohistochemical analysis was performed on trephine bone marrow samples collected from archival material as well as on bone biopsy samples from newly diagnosed patients with MM at Department of Hematology, Faculty of Medicine, Clinical Hospital Centre Rijeka and were subsequently analyzed at Department of Pathology, Faculty of Medicine, University of Rijeka, Croatia.
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student is expected to participate in every aspect of the project. The student will be doing immunohistochemical (IHC) identification and quantification of macrophages and 3D histology specimen reconstruction for precise spatial element recognition (under supervision). The student will also observe the taking of bone biopsies in Clinical Medical Center Rijeka.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
The student will receive preliminary readings to prepare for the project.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Basic knowledge of laboratory work.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months)
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