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Signalling mechanisms regulating adhesion in normal and leukaemia B-cells
Universita degli Studi di Verona
Department of Medicine, Piazzale Ludovico Scuro, Borgo Roma, Verona
Prof. Oliviero Olivieri
Prof. Carlo Laudanna E-mail: firstname.lastname@example.org Phone: 045-8027689 (office); 045-8027145 (lab)
Type of Research Project
- Basic science
What is the background of the project?
Leukocytes recruitment to normal and inflamed tissues is regulated by adhesion locales and chemotactic factors. Complex intracellular signalling networks differently regulate normal versus neoplastic cells transmigration and homing. In the context of leukaemia-derived cells, little is still known about signal transduction mechanisms controlling integrin activation and controlled diapedesis.
What is the aim of the project?
The project aims at defining the specific regulatory role of a class of protein tyrosine kinases and phosphatases in integrin activation by chemokine in normal versus neoplastic B-lymphocytes (B-CLL).
What techniques and methods are used?
In viro adhesion assays under static and under flow conditions. Biochemical analysis on signalling mechanisms. Trojan peptide technology. FPLC protein purification. Cell isolation form CLL patients.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Understanding the logic of complex experiments involving many different techniques and primary human cells. Approaching complex technologies to manipulate signalling events. Analysis of numeric data.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
No theoretical teaching provided
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Ideally, the student should have experience in basic science, especially from the view point of complex experiments. We may provide literature useful to understand the project and the general context.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system
- Montresor A; Toffali L; Mirenda M; Rigo A; Vinante F and Laudanna C. JAK2 tyrosine kinase mediates integrin activation induced by CXCL12 in B cell chronic lymphocytic leukemia. Oncotarget. 2015 Oct 27;6(33):34245-57. PMID: 26413812
- Mirenda M; Toffali L; Montresor A; Scardoni G; Sorio C and Laudanna C. Protein tyrosine phosphatase; receptor type; gamma (PTPRG) is a JAK phosphatase and negatively regulates leukocyte integrin activation. Journal of Immunology 2015 Mar 1;194(5):2168-79 PubMed PMID: 25624455.
- Montresor A; Bolomini-Vittori M; Simon SI; Rigo A; Vinante F; Laudanna C. Comparative analysis of normal versus CLL B-lymphocytes reveals patient-specific variability in signaling mechanisms controlling LFA-1 activation by chemokines. Cancer Res. 2009 Dec 15;69(24): 9281-90. doi: 10.1158/0008-5472.CAN-09-2009. Epub PubMed PMID: 19934331.
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