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Probiotics Interference On Growth And Virulence Of Pathogenic Microorganisms
Universidade de Taubate (UNITAU)
Medicine Department of Univertity of Taubate, Avenida Tiradentes, 500, Taubate, Sao Paulo, Brazilad of the department:
Silvana Soleo Ferreira dos Santos
Silvana Soleo Ferreira dos Santos / Mariella Vieira Pereira Leao
English, Spanish and Portuguese
Type of Research Project
- Basic science
What is the background of the project?
Probiotics are emerging as new strategy to counteract diseases. They are defined as live microorganisms which when used in adequate amounts can confer health benefits (FAO/WHO, 2002). The most common genus with probiotic properties are Lactobacillus and Bifidobacterium, found on intestinal microbiota in a variable manner (Kumar et al. 2016). The prophylactic and therapeutical benefits of probiotics have been clearly reported in different conditions, as infections, allergy, gastrointestinal and metabolic disturbances (Iannitti & Palmieri, 2010; Al Salami et al., 2012) and the manners in which they promote these benefits are mainly by competition for nutrients and for adhesion sites, and production of antimicrobial substances that prevent the multiplication of the pathogen, and/or by improving immune response (Shteyer & Wilschanski, 2008, Gupta & Garg, 2009).
What is the aim of the project?
To investigate the antimicrobial activities of probiotics bacteria, as Lactobacillus, and their influence on the virulence factors expression of pathogenic microorganisms
What techniques and methods are used?
L. rhamnosus (ATCC 1465) or “the pathogen” are cultivated on proper medium and later incubated at 37°C for 24 h. After this period, the culture is centrifuged for 10 min, and the supernatant is discarded. The pellet is resuspended in sterilized saline solution (NaCl 0.9%) and centrifuged again, with the supernatant discarded at another time. This procedure is repeated three times to remove the culture way residues. From the last deposit is prepared standardized suspensions, using spectrophotometry. These suspensions are used in different assays as: agar diffusion, planktonic growth inhibition, inhibition of adhesion on epithelial cells, inhibition of the biofilm formation.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
First, the student will be introduced in the lab. He/she will learn the basic techniques and how to use the main equipment and materials. He /she will also be encourage to read manuscripts regarding the research subject. After, he/she will perform on his/her own: - Writing of the project - Development of proposed assays, in the lab To perform under supervision: - Results interpretation - Statistical analysis - Writing of the manuscript (in case of enough results)
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes. Preliminary reading and learning of the techniques.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster - The student will prepare a presentation - The student will prepare a scientific report - The student will prepare an abstract - The student’s name will be mentioned in a future publication - The student will have the opportunity to present the results together with the supervisor at a conference
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Dedication, practical skills, curiosity, critical thought. Subjects passed: Microbiology
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system
- Reid; G.; Jass; J.; Sebulsky; M. T.; & McCormick; J. K. (2003). Potential Uses of Probiotics in Clinical Practice. Clinical Microbiology Reviews; 16(4); 658–672. http://doi.org/10.1128/CMR.16.4.658-672.2003
- Didari T; Solki S; Mozaffari S; et al. A systematic review of the safety of probiotics. Expert Opinion on Drug Safety. 2014;13(2):227–239.
- Guarner F; Khan AG; Garisch J; et al. World Gastroenterology Organisation Global Guidelines. Probiotics and Prebiotics. October 2011. Journal of Clinical Gastroenterology. 2012;46(6):468–481.
- Sanders ME; Akkermans LMA; Haller D; et al. Safety assessment of probiotics for human use. Gut Microbes. 2010;1(3):164–185.
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