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The role of SIRT1 histone deacetylase in epigenetic modulation of gametes and embryos
Czech Republic (IFMSA CZ) - Charles University in Plzen, Plzen
Biomedical Center, Faculty of Medicine in Pilsen, Charles University, alej Svobody 76, 301 00 Pilsen, Czech Republic
doc. MUDr. Milan Štengl, Ph.D.
Ing. Jan Nevoral Ph.D., MVD. Olga Garcia-Álvarez, Ph.D.
Type of Research Project
- Basic science
What is the background of the project?
Correct epigenetic regulation is crucial for gametogenesis, fertilization and early embryonic development. Endocrine disruptors (EDs), such as bisphenols or xenoestrogens, are able to affect organism without serious clinical expression. However, epigenetics of reproductive processes can be changed and, therefore, EDs influence next generation through epigenetic transgenerational inheritance.
What is the aim of the project?
Tracking of selected epigenetic markers (modified histones, histone deacetylases, histone methyltranferases) in mouse gametes and embryos treated with EDs in vitro - in vivo treatment of mice with EDs and analysis of gametes and embryos - biomonitoring of EDs in human follicular fluid and seminal plasma
What techniques and methods are used?
In vitro oocyte maturation In vitro fertilization Immunocytochemistry Confocal microscopy
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
Student will be working on research project with other members of Laboratory of Reproductive Medicine and, therefore, will become a part of this research team. Basic laboratory skills are expected and required for following designed methodological approaches: manipulation with gametes, embryo culture, immunocytochemistry and microscopy.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
The student will receive materials during preliminary skype interview that is necessary for the student to participate in before being accepted to the project. The articles helpful for the preparation are also mentioned below.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student’s name will be mentioned in a future publication
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Enthusiasm, high motivation to work on the project, self-theoretical preparation and ability to work in a team. Theoretical knowledge about physiology of reproduction, oocyte maturation, fertilization and early embryonic development. Knowledge of molecular mechanism of mentioned processes will be advantaged. Basic laboratory skills for following designed methodological approaches: manipulation with gametes, embryo culture, immunocytochemistry and microscopy.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system
- Moore-Ambriz et al. (2015) Toxicol Appl Pharmacol 289: 507-14. doi: 10.1016/j.taap.2015.10.010
- Tian et al. (2016) Toxicol Ind Health. pii: 0748233716658579. [Epub ahead of print]
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