Role of gut microbiome gene 16SrRNA in the pathogenesis of chronic hepatitis C virus and its complications.
Egypt (IFMSA-Egypt) - Benha Faculty of medicine, Benha
Hepatology, Gastroenterology and infectious diseases department, Benha university hospital, Benha , Egypt
Prof. Mustafa Soliman El-kady
Prof.Reda Muhamed El-Badawy - Dr. Rasha Abd-elhamid El-sayed
4 weeks
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Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Epidemiological studies have revealed that the progression of liver fibrosis in patients with HCV infection is highly variable, including viral co-infection with other viruses e.g. HBV, HIV and host factors e.g gut microbiome gene 16SrRNA, that is related to many of intestinal bacteria. Gut microbiome gene has 150-fold more genes than the human genome as determined with high- throughput DNA, this means a lot of benefit of it.
What is the aim of the project?
To evaluate the role of gut microbiome gene 16SrRNA in stool of patients infected with HCV and it's complications compared with control healthy subjects.
What techniques and methods are used?
prospective cross sectional study for 2 years to all patients infected with hepatitis C with and without complication. The patients will be divided into 2 groups, group 1 with HCV and group 2 control group. The patients will be selected from fever hospital, virology center at Benha after the agreement of the ethical committe. - Stool sample will be collected and processsed accordingly to do culture and to extract microbiome gene 16SrRNA by reverse transcripase PCR. Statistcal analysis will be done to correlate the status of micrbiome with clinical as well as the laboratory and investigations.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
Observation of the lab work (under supervision of the tutor): 1- Stool culture; wet and gram film will be done to detect pus cells and bacteria, then loopful from the sample will be taken to be cultured. The cultured media will be examined for bacterial growth and if mixed growth appear sub culture will be done. The separate bacterial colonies will be identified by growth characters, microscopic examination by gram stain and biochemical reactions. 2- Microbiome detection from stool (16SrRNA gene): stool samples will be used to isolate 16SrRNA by reverse transcriptase PCR in the following steps: 1- DNA extraction 2- reverse transcription 3- DNA amplification 4- DNA detection, - Statistical analysis of the results using SPSS program. Student could do it himself Will there be any theoretical teaching provided for the student (preliminary readings, lectures, courses, seminars etc)? There will be lectures and seminars provided by the tutor about : - 'Hepatitis C virus and it's complicatiion'. - 'The emergent role of gut microbiome gene in the diagnosis and treatment of HCV.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
To be oriented about the basics of statistical analysis
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster
- The student will prepare a presentation
- The student will prepare a scientific report
- The student will prepare an abstract
- The student’s name will be mentioned in a future publication
- The student will have the opportunity to present the results together with the supervisor at a conference
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
What skills are required of the student? To be oriented about the basics of statistical analysis Is there any special knowledge or a certain level of studies needed? Subjects passed: - Physiology - Pathology - Pharmacology - Microbiology - Histology - Biochemistry - Anatomy
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- Tilg H; Cani PD; Mayer EA. Gut microbiome and liver diseases .Gut 2016;65:2035-2044. doi: 10.1136/gutjnl-2016- 312729