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Study the pattern of complications of new antiviral drugs including hepatocellular carcinoma in Chronic hepatits C virus (HCV) patients and associated risk factors.
Egypt (IFMSA-Egypt) - Benha Faculty of medicine, Benha
Hepatology, Gastroenterology and infectious diseases department, Benha university hospital, Benha , Egypt
Prof. Mustafa Soliman El-kady
Prof.Reda Muhamed El-Badawy - Dr. Alaa eldin Ibrahim Attia
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Epidemiological studies revealed that the progression of liver fibrosis to complications like liver cirrhosis, ascites and hepatocellular carcinoma (HCC) in patients exposed to HCV infection is highly variable including viral factors, co-infection with other viruses and host factors including microbiome. Microbiome 16Sr RNA gene emerge as one important host factor needed to be studied. Fatty acid bind protein of liver type (FABPL) emerged as important host factor related to many liver diseases as It plays a pivotal role during intracellular bacterial and viral infections by reducing inflammation and adverse the effect of starvation and energy deficiency on the cell.
What is the aim of the project?
to evaluate the role of Microbiome 16SrRNA gene and Fatty acid bind protein of liver type (FABPL) as important host factors in the pathogenesis of hepatocellular carcinoma (HCC) in HCV patients to avoid the serious complications even after sustained virological response (SVR) and disappearance of virus from the blood.
What techniques and methods are used?
This will be a prospective cross sectional study that will be carried out on 75 patients and 25 control healthy subjects. The patients will be divided into 4 groups; HCV patients with HCC and HCV patients without HCC, patients with HCC but not due to HCV and control subjects with age and Sex matched. All the investigations will be done accordingly ,blood work up, Liver profile, Kidney function tests ,abdominal Ultrasound ,Alfa Fetoprotein and spiral CT for more confirmation . The innovation with the study is detection of fatty acid binding of liver class (FABPL or 1) that will be done by real time polymerase chain reaction (PCR) in blood and also stool analysis for gut microbiome Gene 16SrRNA ,it will be done by DNA sequestration.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
Data collection: collecting medical history from subjects (HCV patients with HCC and HCV patients without HCC, patients with HCC but not due to HCV and control subjects with age and Sex matched.) - Observation of the lab work: Microbiome detection from Stool(16SrRNAgene) byreverse transcriptase PCR in the following steps:- 1- DNA extraction 2- reverse transcription 3- DNA amplification 4- DNA detection. - Statistical analysis of the results
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
There will be lectures and seminars provided by the tutor.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster - The student will prepare a presentation - The student will prepare a scientific report - The student will prepare an abstract - The student’s name will be mentioned in a future publication - The student will have the opportunity to present the results together with the supervisor at a conference
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
To be oriented about the basics of statistical analysis Is there any special knowledge or a certain level of studies needed? Subjects passed: - Physiology - Pathology - Pharmacology - Microbiology - Histology - Biochemistry - Anatomy
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months)
- Circulating adipocyte-fatty acid binding protein levels predict the development of the metabolic syndrome: a 5-year prospective study. Xu A et al. Circulation. (2007) DOI: 10.1161/CIRCULATIONAHA.106.647503
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