Projects
Name
The prevention of diabetes-induce damage of dopaminergic neurons in substantia nigra by polyunsaturated fatty acids in rat model of streptozotocin diabetes mellitus
University
Croatia (CroMSIC) - University of Split, Split
Domain
Anatomy
Departement
Laboratory for Neurocardiology, Department of Anatomy, Histology and Embryology
Head
Natalija Filipović, PhD, DVM , Katarina Vukojević, PhD, MD
Tutor
): Natalija Filipović, PhD, DVM
Languages
English
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No No Yes No No No No No
Type of Research Project
- Basic science
What is the background of the project?
It has been reported that 50% to 80% of patients with Parkinson's disease have abnormal glucose tolerance which may be further exacerbated by levodopa therapy. Little is known about the impact of chronic hyperglycemia on the severity of the motor manifestations and the course of the disease. In this project, In the rat model of streptozotocin induced diabetes mellitus (DM), this connection is being studied, as well as the influence of supplementation with poliunsaturated fatty acids on damage of dopaminergic neurons in substantia nigra.
What is the aim of the project?
The purpose is to improve knowledge about the connection between diabetes mellitus and Parkinson disease, which was established recently in systematic reviews. We also explore the possibility to prevent these changes by supplementation with polyunsaturated fatty acids. We are using histology and imunohistochemistry as main methods to research damage of neurons in substantia nigra and changes in different growth factors, receptors , glia infiltration and activation, dynamics of lipid droplets in glia and morphological signs of decay of neurons. The experimental part with animals is already finished and samples of brain are collected.
What techniques and methods are used?
We are using histology and imunohistochemistry as main methods to research damage of neurons in substantia nigra and changes in different growth factors, receptors , glia infiltration and activation, dynamics of lipid droplets in glia and morphological signs of decay of neurons. The experimental part with animals is already finished and samples of brain are collected.
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Tutor expects that student will take part in preparation of samples – embedding in paraffin wax, cutting, immunohistochemical staining, microscopy and capturing of microphotographs, by using of bright-field and fluorescent microscope, and participate in analyses of microphotographs. Also highly motivated student could expect to be introduced in basic statistical analyses of the obtained data and preparation of plates and figures for the purposes of publication, by using Photoshop.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
All parts of the process – embedding in paraffin wax, cutting, immunohistochemical staining, microscopy and capturing of microphotographs, by using of bright-field and fluorescent microscope, analyses of microphotographs by ImageJ programme, basics of statistical analyses work with Photoshop programme will be demonstrated and explained to the student by myself or my collaborators in the lab. The previous knowledge is an advantage, but is not necessary.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
All parts of the process will be demonstrated and explained to the student by myself or my collaborators in the lab. The previous knowledge is an advantage, but is not necessary
Are there any legal limitations in the student’s involvement
No
Hours
7
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
Articles