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(Temuco) Molecular characterization of the anti-inflammatory activity of berberinicos alkaloids associated with the satellite signal transduction TLR4/NF-kβ in murine monocytes activated with LPS
Universidad de la Frontera
Departament of Basic Sciences, Faculty of Medicine
Marco Paredes H, PhD
Marco Paredes H, PhD
English and Spanish
Type of Research Project
- Basic science
What is the background of the project?
In Chile there are a large variety of native plant species used by local ethnicities for the treatment of various diseases. One of these plants known as "Michay" has historically been used by Mapuche for the treatment of fever, inflammation, stomach pain, indigestion and diarrhea. However, to date, there are no studies demonstrating its anti-inflammatory properties. Therapeutic effects associated with these plants appear to be related to the existence of secondary metabolites, where alkaloids are the most abundant and diverse in this plant. Our previous work shows that alkaloids extracted from the michay root inhibit functional responses in LPS-activated monocytes in vitro. These results suggest that the TLR4 / NF-kB signal translation pathway could be inhibited. What is the aim of the project? Previous work in our laboratory shows that some functional berberinicos alkaloids inhibit responses in monocytes activated by LPS in vitro. These results suggest that the transcription factor NF-kB may be affected in its functional role. A evidence that supports this hypothesis in activated monocytes with lipopolysaccharide (LPS) and treated with berberinicos alkaloids inhibits the translocation of this factor to the nucleus. Techniques: 1.- Cell culture 2.- Immunofluorescence 3.- Confocal microscopy
What is the aim of the project?
Previous work in our laboratory shows that some functional berberinicos alkaloids inhibit responses in monocytes activated by LPS in vitro. These results suggest that the transcription factor NF-kB may be affected in its functional role. A evidence that supports this hypothesis in activated monocytes with lipopolysaccharide (LPS) and treated with berberinicos alkaloids inhibits the translocation of this factor to the nucleus.
What techniques and methods are used?
1.- Cell culture 2.- Immunofluorescence 3.- Confocal microscopy
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will join a scientific working group in which he will collaborate in research work and experimental activities supervised by the laboratory chief. He will also participate in various academic activities such as: seminars, thesis presentation and discussion of results.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
1.- TLR4 / NF-kB signal transduction pathway in monocytes / macrophages 2.- Immunofluorescence 3.- Confocal microscopy
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student’s name will be mentioned in a future publication
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Requirements: - lab coat - experience in cell biology lab - Cell Biology subject passed
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Students in biomedical fields
- Taro Kawai and Shizuo Akira (2007). Signaling to NF-kB by Toll-like Receptors TRENDS in Molecular Medicine Vol.13 No.11
- Lien E; Means TK; Heine H;Yoshimura A; Kusumoto S; et al. 2000.Toll-like receptor 4 imparts ligand-specific recognition of bacterial lipopolysaccharide. J. Clin. Invest. 105:497–504
- Imanshahidi M; Hosseinzadeh H (2008). Pharmacological and therapeutic effects of Berberis vulgaris and its active constituent; berberine. Phytother Res 22: 999-1012.
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