Evaluation of mutation/polymorphism in candidate gene in infertile women
Faculdade de Medicina do ABC (FMABC)
Reproductive Medicine
Department of Collective Health of Faculdade de Medicina do ABC (FMABC) on 821 Principe de Gales Avenue, Santo Andre ? SP, Brazil. Postal Code 09060-650.
Professor Vania Barbosa
Professor Bianca Bianco
English, Spanish, Italian and Portuguese
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No No No Yes No No No No
Type of Research Project
- Basic science
What is the background of the project?
In assisted human reproduction, response to controlled ovarian hyperstimulation is variable and difficult to predict. In young ovulatory women undergoing in vitro fertilization (IVF) protocol standard stimulation can result in both satisfactory answer, as for inadequate response which requires dose adjustment of Follicle Stimulating Hormone (FSH) or ovarian hyperstimulation syndrome, a serious and potentially fatal complication IVF. Identifying patients with potential to develop hyper-response or inadequate response to standard treatment would be of great clinical aid. Currently, basal FSH on the third day of the cycle seems to have the best predictive ability. Moreover, it has been suggested that mutations and polymorphisms in the FSHR gene may cause the arrest of follicular growth leading to decreased ovarian reserve. Since these genes are expressed during oogenesis, its mutations can cause varying degrees of blockage in the formation of germ cells. Small variations in these genes might determine the variability of follicle pool and thus account for the variability of response to ovarian stimulation and outcome of assisted reproduction. These findings confirm the important role of kisspeptin in the physiological regulation of the hypothalamic-pituitary-gonadal axis in mammals.
What is the aim of the project?
To identify candidate genes that can predict ovarian hyperstimulation response and human reproduction outcomes.
What techniques and methods are used?
Real time Polymerase Chain Reaction (RT-PCR), DNA extraction.
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The main point is to acknowledge on the routine of a Human Reproduction service, its difficulties and also to get to know the routine of a Genetics Lab service associated to Human Reproduction. The student will acquire skills on Clinical and Laboratory activities such as DNA extraction, RT-PCR, etc.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
It is desirable that the student had passed/is coursing both Genetics and Gynaecology courses.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- PABALAN; NOEL; MONTAGNA; ERIK; SINGIAN; ELOISA; TABANGAY; LANI; JARJANAZI; HANNDI; BARBOSA; CAIO PARENTE; BIANCO; BIANCA. Associations of Polymorphisms in Anti-Mullerian Hormone (AMH Ile49Ser) and its Type II Receptor (AMHRII-482 A > G) on Reproductive Outcomes and Polycystic Ovary Syndrome: a Systematic Review and Meta-Analysis. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY; v. 39; n. 6; p. 2249-2261; 2016. Web of Science Citations: 0.
- PELUSO; CARLA; FONSECA; FERNANDO L. A.; GASTALDO; GUILHERME G.; CHRISTOFOLINI; DENISE M.; CORDTS; EMERSON BARCHI; BARBOSA; CAIO P.; BIANCO; BIANCA. AMH and AMHR2 Polymorphisms and AMH Serum Level Can Predict Assisted Reproduction Outcomes: A Cross-Sectional Study. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY; v. 35; n. 4; p. 1401-1412; 2015. Web of Science Citations: 8.
- DE MATTOS; CLARISSA SANTIAGO; TREVISAN; CAMILA MARTINS; PELUSO; CARLA; ADAMI; FERNANDO; CORDTS; EMERSON BARCHI; CHRISTOFOLINI; DENISE MARIA; BARBOSA; CAIO PARENTE; BIANCO; BIANCA. ESR1 and ESR2 gene polymorphisms are associated with human reproduction outcomes in Brazilian women. JOURNAL OF OVARIAN RESEARCH; v. 7; DEC 20 2014. Web of Science Citations: 3.
- PABALAN; NOEL; TREVISAN; CAMILA MARTINS; PELUSO; CARLA; JARJANAZI; HAMDI; CHRISTOFOLINI; DENISE MARIA; BARBOSA; CAIO PARENTE; BIANCO; BIANCA. Evaluating influence of the genotypes in the follicle-stimulating hormone receptor (FSHR) Ser680Asn (rs6166) polymorphism on poor and hyper-responders to ovarian stimulation: a meta-analysis. JOURNAL OF OVARIAN RESEARCH; v. 7; n. 122 DEC 20 2014. Web of Science Citations: 2