Projects
Name
Acute oral toxicity, mutagenicity, genotoxicity and embryotoxicity of Ruthenium (II) complexes in vitro and in vivo assay for the treatment of cancer.
University
Federal University of Goiania (UFG)
Domain
Genetics
Departement
Laboratory of Molecular Genetics and Cytogenetics, Institute of Biological Sciences, Department of Genetics, Federal University of Goias, Goiania, GO, 74690-900, Brazil.
Head
Elisangela de Paula Silveira Lacerda
Tutor
Elisangela de Paula Silveira Lacerda
Languages
portuguese, english
Duration
8 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No yes yes yes yes yes No yes yes yes yes yes
Type of Research Project
- Basic science
What is the background of the project?
In studies for the development of new drugs to combat cancer, two have stood out due to their advance to clinical trials: NAMI-A (imidazolium [trans-imidazoledimethylsulfoxide-tetrachlororuthenate (III)]) and KP1019 (indazolium [trans-tetrachlorobis(1H-indazole) ruthenate(III)]). However, these substances presented several adverse effects leading to new ruthenium complexes, especially type II, synthesis and studies, aiming to find a compound less toxic to be used in cancer treatment.
What is the aim of the project?
What is the aim of the project? Find new cancer treatment drugs studying the anticancer potential of ruthenium (II) complexes and investigating the cytotoxicity, genotoxicity and toxicity profile of this complexes.
What techniques and methods are used?
What techniques and methods are used: This project involves two parts: (1) in vitro experiments, that includes cell culture, cell viability, comet and micronucleus assay, cell cycle analysis, total RNA extraction and cDNA synthesis, quantitative real-time reverse transcriptase, analysis of mitochondrial membrane potential, assessment of intracellular Caspase-3 activities, Bcl-2 analysis by flow cytometry; and (2) in vivo experiments, that includes molecular florescence tomography (to describe the tumor progression), histopathological examination of the tumor, acute oral toxicity study in rats and statistical analysis of the results.
What is the role of the student?
What are the tasks expected to be accomplished by the student?
This project involves two parts: (1) in vitro experiments, that includes cell culture, cell viability, comet and micronucleus assay, cell cycle analysis, total RNA extraction and cDNA synthesis, quantitative real-time reverse transcriptase, analysis of mitochondrial membrane potential, assessment of intracellular Caspase-3 activities, Bcl-2 analysis by flow cytometry; and (2) in vivo experiments, that includes molecular florescence tomography (to describe the tumor progression), histopathological examination of the tumor, acute oral toxicity study in rats and statistical analysis of the results.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
1
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
The student need to be able to discuss cell cycle, cell culture and cancer mechanism. Is there any special knowledge or a certain level of studies needed? Subjects passed: molecular biology, cell biology. Previous experience with: pipetting and preparing of solutions.
Are there any legal limitations in the student’s involvement
No
Hours
6
Type of students accepted
This project accepts:
- Medical students
- Pre-Medical students from the American-British system
Articles
- Pereira FC; Lima BAV; Lima AP; Pires WC.; Monteiro T; Magalhaes LF.; Costa W; Graminha AE; Batista AA; Ellena J; Lacerda EPS. Cis-[RuCl(BzCN)(N?N)(P?P)]PF6 complexes: Synthesis and in vitro antitumor activity: (BzCN = benzonitrile; N?N = 2;2