Projects
Name
Effects of sodium overload in post natal phase in rats on the hydroelectrolytic balance.
University
Federal University of Goiania (UFG)
Domain
Physiology
Departement
Department of Physiological Sciences, Av. Esperan
Head
Daniel A. Rosa
Tutor
Andre H. Freiria-Oliveira, Ph. D., Aryanne B. S. Melo Ph. D. student
Languages
portuguese, english
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
yes yes yes yes yes yes No yes yes yes yes No
Type of Research Project
- Basic science
What is the background of the project?
Plasma osmolality changes can be too dangerous to a healthy life. Many studies have showed that hyperosmolality can influence blood pressure and inflammation process. Vidonho et al (2004) showed that offspring of mothers that was exposed to a high salt diet during pregnancy and lactation presented high blood pressure levels in adult life. However, the effects of this diet in young life phase, after lactation, is poorly understood yet.
What is the aim of the project?
What is the aim of the project? We investigate how overload of salt in the young life of rats, after lactation, could impact the water and sodium intake and urinary excretion in adult phase.
What techniques and methods are used?
What techniques and methods are used: We use Wistar male rats and exposed as the only fluid source a 0,3 M NaCl solution after lactation (21 days) during 60 days. After the treatment, the animals are kept in a recovery period (15 days) with tap water. After that they are maintained in individual cages with water and 0,3 M NaCl bottles, and the fluid are measured daily. After 5-7 days, dehydration protocols are performed to investigate hydroelectrolytic balance. The animals are allocated in metabolic cages and an intragastric 2 M NaCl load (2 mL, an intracellular dehydration) are performed by gavage. One hour later, glass burettes contained water and 0,3M NaCl were offered and the intake was measured by 2 hours. Urinary salmples are collected before and after fluid intake. A week later, other protocol are performed, 24 hours of water deprivation (an extracellular dehydration). The animals are kept in metabolic cages during 24 hours without access to water, after this period an urinary sample is collected and water and 0,3 M NaCl burettes are offered and the intake is measured through 2 hours. At the end another urinary sample is collected. Urinary volume, osmolality and sodium excretion are calculated using an osmometer and a flame photometer.
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
What techniques and methods are used: We use Wistar male rats and exposed as the only fluid source a 0,3 M NaCl solution after lactation (21 days) during 60 days. After the treatment, the animals are kept in a recovery period (15 days) with tap water. After that they are maintained in individual cages with water and 0,3 M NaCl bottles, and the fluid are measured daily. After 5-7 days, dehydration protocols are performed to investigate hydroelectrolytic balance. The animals are allocated in metabolic cages and an intragastric 2 M NaCl load (2 mL, an intracellular dehydration) are performed by gavage. One hour later, glass burettes contained water and 0,3M NaCl were offered and the intake was measured by 2 hours. Urinary salmples are collected before and after fluid intake. A week later, other protocol are performed, 24 hours of water deprivation (an extracellular dehydration). The animals are kept in metabolic cages during 24 hours without access to water, after this period an urinary sample is collected and water and 0,3 M NaCl burettes are offered and the intake is measured through 2 hours. At the end another urinary sample is collected. Urinary volume, osmolality and sodium excretion are calculated using an osmometer and a flame photometer.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
0
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Being open to new culture, discussing subjects related to the project and the area of research in the laboratory, being an extrovert.
Are there any legal limitations in the student’s involvement
No
Hours
6
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
- Pre-Medical students from the American-British system
Articles
- ANTUNES-RODRIGUES; J.; DE; C. M.; ELIAS; L. L.; VALENCA; M. M.; MCCANN; S. M. Neuroendocrine control of body fluid metabolism. Physiol Rev.; v. 84; n. 1; p. 169-208; 2004.
- BLANCH GT; FREIRIA-OLIVEIRA AH; MURPHY D; PAULIN RF; ANTUNES-RODRIGUES J; COLOMBARI E; et al. Inhibitory mechanism of the nucleus of the solitary tract involved in the control of cardiovascular; dipsogenic; hormonal; and renal responses to hyperosmolality. AJP Regul Integr Comp Physiol. 2013 Jan 30; 304(7):R531?42.
- BOURQUE; C. W. Central mechanisms of osmosensation and systemic osmoregulation. Nat.Rev.Neurosci.; v. 9; n. 7; p. 519-531; 2008.
- FITZSIMONS; J. T. Angiotensin; thirst; and sodium appetite. Physiological Reviews; v. 78; n. 3; p. 585-686; 1998.
- MACHADO; B. H. Neurotransmission of the cardiovascular reflexes in the nucleus tractus solitarii of awake rats. Ann.N.Y.Acad.Sci.; v. 940; p. 179-196; 2001.
- MCKINLEY; M. J.; ALLEN; A. M.; MAY; C. N.; MCALLEN; R. M.; OLDFIELD; B. J.; SLY; D. et al. Neural pathways from the lamina terminalis influencing cardiovascular and body fluid homeostasis. Clin.Exp.Pharmacol.Physiol; v. 28; n. 12; p. 990-992; 2001
- MCKINLEY; M. J. ; JOHNSON; A. K. The physiological regulation of thirst and fluid intake. News Physiol Sci.; v. 19; p. 1-6; 2004.
- STRICKER; E. M. ; SVED; A. F. Thirst. Nutrition; v. 16; n. 10; p. 821-826; 2000.