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(Antofagasta) Evaluation of antioxidants properties of exopolysaccharides from cyanobacteria
Universidad de Antofagasta
Biochemistry Laboratory , Biomedical Department
Prof. Luis Urrutia
Prof. Alexandra Galetovic Carabantes
English, Spanish, Portuguese
Type of Research Project
- Basic science
What is the background of the project?
This project is within our research line about isolation and biochemical identification of molecules with biotechnological interest from microorganisms of the Atacama Desert. Our laboratory works with cyanobacteria and we are interested in studying antioxidants properties of biomolecules from cyanobacteria. We have one strain that produce high content of exopolysaccharides in laboratory conditions and we would like to determinate antioxidants properties of sugars and pigments for potential biotechnological purposes. aim: To study antioxidants properties of exopolysacharides Techniques: Classicals methods to determinate antioxidant properties based on chemical reactions, for example, FRAP (Ferric Reducing Ability of Plasma) and ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid radical cation.
What is the aim of the project?
To study antioxidants properties of exopolysacharides.
What techniques and methods are used?
Classicals methods to determinate antioxidant properties based on chemical reactions, for example, FRAP (Ferric Reducing Ability of Plasma) and ABTS (2,2'-azino- bis(3- ethylbenzothiazoline-6- sulphonic acid radical cation.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
The student has to be done under supervision the following tasks: -To prepare culture medium for cyanobacteria. -To cultivate cyanobacteria under laboratory conditions. -To purify exopolysaccharides. - To apply standard protocols for the determination of antioxidant properties of exopolysaccharides.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
There will be preliminary readings about cianobacteria and exopolysaccharides.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a scientific report - The student’s name will be mentioned in a future publication
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Responsability ans dedication.Previous experience with: solution preparation, to calculate concentrations.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system
- Re; R.; Pellegrini;N.; Proteggente;A.; Pannala; A.; Yang;M. and Rice-Evans; C.(1999). Antioxidant Activity Applying an Improved ABTS Radical Cation Decolorization Assay. Free Radical Biology & Medicine; Vol. 26; Nos. 9/10; pp. 1231?1237.
- - Benzie I and Strain J. The Ferric Reducing Ability of Plasma (FRAP) as aMeasure of Antioxidant Power: The FRAP Assay. Analytical Biochemistry 239; 70-76 (1996).
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