Projects
Name
Microenvironment factors in the prognosis of prostate cancer
University
Croatia (CroMSIC) - University of Rijeka, Rijeka
Domain
Pathology
Departement
Department of pathology
Head
Nives Jonjić M.D., Ph.D.
Tutor
Gordana Đorđević M.D., Ph.D.
Languages
English
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No No Yes Yes No No No No
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Prostate cancer is the most common malignancy and the second most frequent cause of death among men. The choice of therapy according to the latest criteria of aggressiveness and risk recurrence based still on the classical clinicopathological factors. Recent research based on changes of the microenvironment and tumor infiltrating lymphocytes (TIL's) which can initiate progression of cancer. The remodeling of the extracellular matrix (ECM) and epithelial mesenchymal transition (EMT) starts with a reduced expression of adhesion molecules, increased expression of matrix metalloproteinases (MMPs), which is provoked especially by the action of chemokines, among others interleukin-8 (IL-8). Previous retrospective studies ECM changes in prostate cancer within the project "Molecular mechanisms of tumor expression and metastasis of breast cancer and prostate cancer" (062-0620095-0077), included the importance of tissue expression of MMP 2 and 9, CD44 and Ki67 molecules in prostate cancer prognosis. The study of molecular biomarkers p53, NFkB, bcl 2 contributed to the knowledge of the importance of the different expression of cell cycle regulators for the behavior of prostate cancer in the moment of radiotherapy. Planned continuation of this work should compare these molecules in different groups of patients in relation to the therapy. Planned continuation of this work should compare these molecules in different groups of patients in relation to the therapy. According to the risk guidelines for patients with localized disease, our prospective study is based on the expectation that the determination of IL-8 and MMP 9 in the serum could indicate a moment of tumor progression and the need for more aggressive therapies. We expect that the quantification and the characterization of TIL, along with clinical pathological parameters, could be used as a simple clinical index in predicting recurrence based on the immune response of the tumor. Our prospective study is based on the expectation that the determination of IL-8 and MMP 9 in the serum could indicate a moment of tumor progression and the need for more aggressive therapies. We expect that the quantification and the characterization of TIL, along with clinical pathological parameters, could be used as a simple clinical index in predicting recurrence based on the immune response of the tumor
What is the aim of the project?
Planned continuation of this work should compare these molecules in different groups of patients in relation to the therapy. Planned continuation of this work should compare these molecules in different groups of patients in relation to the therapy. According to the risk guidelines for  patients with localized disease, our prospective study is based on the expectation that the determination of IL-8 and MMP 9 in the serum could indicate a moment of tumor progression and the need for more aggressive therapies. We expect that the quantification and the characterization  of TIL, along with clinical pathological parameters, could be used as a simple clinical index in predicting recurrence based on the immune response of the tumor.
What techniques and methods are used?
Our prospective study is based on the expectation that the determination of IL-8 and MMP 9 in the serum could indicate a moment of tumor progression and the need for more aggressive therapies. We expect that the quantification and the characterization  of TIL, along with clinical pathological parameters, could be used as a simple clinical index in predicting recurrence based on the immune response of the tumor.
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student is expected to participate in every aspect of the project. There will be some clinical work (mostly taking blood samples) which will be done by the student under observation from the professor. After that the student will measure the levels of IL-8 and MMP-9 and also some other clincally important markers that will be useful in the advancement of the project. Other parts of the project in which the student will participate will be explained by the professor.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
The student will receive some preliminary readings to prepare for the project.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Basic knowledge of laboratory work.
Are there any legal limitations in the student’s involvement
No
Hours
6
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
Articles
- "Molecular mechanisms of tumor expression and metastasis of breast cancer and prostate cancer" (062-0620095-0077)