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The study of the molecular mechanisms of immunomodulatory and antitumor effect of a probiotic strain Lactobacillus plantarum LS/07 and bioactive compounds of natural origin in an in vitro model of colon adenocarcinoma.
University of Pavol Jozef Safarik
Department of experimental Medicine,
MVDr. Alojz Bomba, DrSc.
RNDr. Jana Stofilova, PhD.
Type of Research Project
- Basic science
What is the background of the project?
Some strains of probiotic bacteria have immunoregulatory and antitumor effects as demonstrated by several experimental studies in vivo and in vitro, showing a cytotoxic and inhibitory effect on the development and progression of colon cancer by modulating cellular and humoral immunity. The exact mechanism of this effect remains still not elucidated. Our previous results suggest that the substances of natural origin (prebiotic oligofructose and flaxseed oil rich in n-3 PUFAs) may potentiate the anticancer effect of probiotic lactobacilli during chemically induced colon carcinogenesis. The current project is focused on molecular mechanisms of immunomodulatory and antitumor effects of the specific probiotic strain Lactobacillus plantarum LS/07 alone or in combination with selected bioactive compounds in an in vitro model of colon adenocarcinoma.
What is the aim of the project?
The main objective of this project is to acquire new knowledge about the molecular mechanisms of immunomodulatory and antitumor effects of probiotic strain Lactobacillus plantarum LS/07 and bioactive natural substances in vitro in model of colon adenocarcinoma.
What techniques and methods are used?
Cell culture techniques, basic microbiological cultivation techniques, cell viability assays, flow cytometry, molecular techniques (RNA isolation, real time PCR ?Polymerase Chain Reaction) and immunological techniques such as ELISA (The Enzyme-Linked Immunosorbent Assay) method.
What is the role of the student?
What are the tasks expected to be accomplished by the student?
interest , participation
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
The student should have already studied preclinical subjects such as cytology, molecular biology, biochemistry and microbiology. The main requirement is an active approach during the research fellowship. Subjects passed: cytology, molecular biology, immunology. Previous basic experience in laboratory are desirable.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
- Bomba A. et al. The possibilities of potentiating the efficacy of probiotics. Trends Food Sci Tech 2002; 13: 121-126
- Kahouli I. et al. Probiotics in colorectal cancer (CRC) with emphasis on mechanisms of action and current perspectives. J Med Microbiol 2013; 62: 1107-1123
- ATCC Animal Cell Culture Guide Tips and Techniques for Continuous Cell Lines
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