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The dependence between the representation the PUFA and LBP /sCD14 ratio in blood serum in patients with breast cancer
University of Pavol Jozef Safarik
Institute of Experimental Medicine
MVDr. Alojz Bomba, DrSc.
RNDr. Zdenka Hertelyova, PhD.
English, Polish, Czech
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Breast cancer is the most common cancer disease among women in economically developed countries. The exact cause of the disease is not fully understood, but a variety of risk factors applies here. Consequently, the prevention has an important role in the reduction of incidence of breast cancer. Residents of Central and Western Europe consume food with increased content of omega-6 at the expense of omega-3 polyunsaturated fatty acids (PUFA). Consumption of high-fat diet rich in saturated fatty acids is associated with changes in the proportions of bacterial genera and species of intestinal microflora. In this way there is development of dysbiosis.
What is the aim of the project?
. The mutual disproportions between the fatty acids, as well as dysbiosis, are risk factors, but also causes of the development of many chronic diseases, too. The present proposal aims to map and assess the ratio of omega-6/omega- 3, to examine the interdependence of the PUFA and LBP/sCD14 (Lipopolysaccharide binding protein /Cluster of Differentation 14) ratio and the role of intestinal microflora in the pathogenesis of breast cancer. Monitor and compare the presence of polyunsaturated fatty acids and their ratios for groups of subjects: healthy women, obese women (increased BMI ? Body Mass Index and waist circumference), non-obese women with breast cancer and obese women with breast cancer. Monitor the response of the organism to passage of LPS - Lipopolysaccharides (antigen of the Gram negative bacteria) by assessing LBP to sCD14 ratio in selected groups of subjects. Assess the role of the intestinal microflora and PUFA in the pathogenesis of breast cancer. Outline possibilities approaches for prevention of breast cancer.
What techniques and methods are used?
ELISA (The Enzyme-Linked Immunosorbent Assay) methods, Biochemical analyzer RX Daytona 3901 ? biochemical parameters ? metabolic, lipid and Gas chromatography with mass spectrometry and flame-ionization detector (GC-FID Agilent 7890 A, mass spectrometer 5975 C, column capillary DB 23).
What is the role of the student?
What are the tasks expected to be accomplished by the student?
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
To have a desire to work in the laboratory.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months)
- Fergusson J.F. et al. (2014): Omega-3 polyunsaturated fatty acid supplementation and the response to evoked endotoxemia in healthy volunteers. In:?Mol Nutr Food Res. 2014. 58 (3): 601?613
- Cani P.D. et al. (2009). Interplay between obesity and associated metabolic disorders: new insights into the gut microbiota. In:?Curr Opin Pharmacol.?2009. 9 (6):737-743
- Xue F; Michels KB. (2007). Diabetes; metabolic syndrome; and breast cancer: a review of the current evidence. In:?Am J Clin Nutr. 2007. 86 (3): 823-835
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