The effects of melatonin on hepatic regeneration after partial hepatectomy in young and aged sprague-dawley rats
Turkey (TurkMSIC) - Bezmialem University, Istanbul
Department of Histology and Embryology
Prof. Dr. Mukaddes Esrefoğlu
Prof. Dr. Mukaddes Esrefoğlu E-mail:
English, Turkish
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No Yes Yes Yes No No No No
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
The liver is a unique organ that actually can regenerate itself. Following an injury, ratio of hepatocytes entering S phase is 90-100% in young whereas 30% in old persons. Researches related to liver regeneration are generally designed by performing partial hepatectomy (PH). Liver regeneration after partial hepatectomy is a very complex and well-orchestrated phenomenon. It is carried out by the participation of all mature liver cell types. The process is associated with signaling cascades involving growth factors, cytokines, matrix remodeling, and several feedbacks of stimulation and inhibition of growth related signals. Liver manages to restore any lost mass and adjust its size to that of the organism, while at the same time providing full support for body homeostasis during the entire regenerative process. In previous studies it is showed that melatonin decreased hepatic injury induced by CCl4 (carbon tetrachloride) , AA (amino acid) and CCl4 + AA. The inhibition of the oxidative stress and therefore lipid peroxidation by melatonin in CCl4 and AA administered animals, may constitute the protective mechanism of melatonin against acute liver injury. he aim of this study is to investigate hepatic regeneration capacity following PH in young and aged rats and the effects of melatonin on this process. In our study, morphological characteristic of liver (weight and size), proliferative (PCNA - Proliferating cell nuclear antigen) and apopitotic (TUNEL Terminal Uridine Nick-End Labeling ) cell numbers, distribution and number of stem/progenitor cells (OV6+ [oval cell marker antibody] for hepatic progenitor cells), hepatoblasts (AFP+ [alpha-fetoprotein]) by confocal microscopy, tissue levels of CK19 (cytokeratin 19), TNF-α (tumor necrosis factor alpha), TGF-β (transforming growth factor beta), IL-6 (interleukin 6), EGF (epiderml growth factor), VEGF (vascular endothelial growth factor), HGF (hepatocyte growth factor) by Western blot analysis, levels of plasma oxidants and antioxidants, DNA damage levels in mononuclear leukocytes, serum levels of ALT (Alanine Aminotransferase) , AST (Aspartate Aminotransferase) and ALP (Alkaline Phosphatase) were investigated.
What is the aim of the project?
What techniques and methods are used?
What is the role of the student?
What are the tasks expected to be accomplished by the student?
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
We only expect the student to be hardworking and passionate about science.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students