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Confronting immunohistochemistry ATRX, IDH1 and P53 profiles with co- delection 1p19q in low grade diffuse gliomas
Portugal (PorMSIC) - University of Lisbon, Lisbon
Laboratory of Neuropathology, Department of Neurosciences, Hospital de Santa Maria (CHLN), Av. Prof. Egas Moniz 1649-036 Lisbon, Portugal
Jose Pimentel, MD, PhD
Rafael Roque, MD Pedro Pereira, MsC
English, Spanish, French
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
In the last years, the diagnosis of gliomas has been challenged by the need of searching protein mutations, either by immunohistochemistry or genetics, which are now critical for both their treatment and prognosis. In the routine of most departments of neuropathology, the use of antibodies against ATRX (ATP-dependent helicase ATRX), IDH1 (Isocitrate dehydrogenase 1) (plus the older P53 mutation) and the searching of co-delection 1p19q are currently mandatory, which allows the reclassification of gliomas, namely the diffuse ones.
What is the aim of the project?
In a sample of diffuse, low grade gliomas diagnosed by conventional morphology, we aim to confront the value of the immunohistochemistry ATRX, IDH1 and P53 data with the presence/absence of the co-delection 1p19q (performed by Fluorescence in situ hybridization (FISH) method) for the diagnosis.
What techniques and methods are used?
We will watch the tumors on the microscopy and diagnose them. We will use immunohistochemistry techniques to search for protein mutations (ATRX, IDH1 and P53) and we will use FISH to the search for co-delection 1p19q.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
To watch the tumors on the microscopy and to learn how to diagnose them; To learn how to perform the immunohistochemistry and FISH techniques; To review the most important publications on the issue; To start writing a report about the research and to idealize a publication/presentation. All work will be done with supervision. However, depending on the experience and understanding about the techniques, the student will be able to perform them alone.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes, lectures and preliminary readings.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation - The student will prepare an abstract - The student’s name will be mentioned in a future publication
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
To interested in neuropathology, mainly on central nervous system primitive tumors, and to like to watch a microscope
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months)
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- J Mol Diagn. 2016 Sep;18(5):620-34. doi: 10.1016/j.jmoldx.2016.05.005. Recent Advances on the Molecular Pathology of Glial Neoplasms in Children and Adults. Rodriguez FJ1; Vizcaino MA2; Lin MT3.
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- Handb Clin Neurol. 2016;134:361-80. doi: 10.1016/B978-0- 12-802997- 8.00022-0. Low-grade and anaplastic oligodendroglioma. Van Den Bent MJ1; Bromberg JE2; Buckner J3.
- Jpn J Clin Oncol. 2017 Jan;47(1):2-6. doi: 10.1093/jjco/hyw142. Epub 2016 Oct 31. Classification of adult diffuse gliomas by molecular markers-a short review with historical footnote. Otani R1; Uzuka T1; Ueki K2;3.
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