Projects
Name
Mechanistic investigation of kidney injury
University
University of British Columbia
Domain
Urology
Departement
Urological Sciences
Head
Dr. Christopher Nguan
Tutor
Caigan Du
Languages
English
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
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Type of Research Project
- Basic science
What is the background of the project?
Background: Ischemia Kidney injury occurs during kidney transplantation and is a common problem in nephrology clinic. Our research project is mainly related to the mechanistic investigation of kidney ischemia-reperfusion injury using kidney cell cultures and mouse model. Currently we are trying to understand the pathways mediating hypoxia-mediated kidney epithelial cell death, cellular pathways for kidney tissue repair and the mechanisms underlying kidney inflammation after kidney ischemia-reperfusion injury. Aim: To understand the molecular and cellular pathways mediating kidney cell death and repair/regeneration. Technique: 1) Cell Cultures: you will learn how to grow cells in both 2-D and 3-D cultures, and learn how to identify cell death using a flow cytometry or other methods, such as microscopy and Western blot. 2) PCR array, microarry and bioinformatics analysis: you will learn how to extract RNA from cells/tissues and perform the PCRarray and/or microarray to see the transcriptome in collaboration with molecular biology lab, and also perform pathway analysis using bioinformatics tool with our bioinformatics group.
What is the aim of the project?
To understand the molecular and cellular pathways mediating kidney cell death and repair/regeneration.
What techniques and methods are used?
1) Cell Cultures: you will learn how to grow cells in both 2-D and 3-D cultures, and learn how to identify cell death using a flow cytometry or other methods, such as microscopy and Western blot. 2) PCR array, microarry and bioinformatics analysis: you will learn how to extract RNA from cells/tissues and perform the PCRarray and/or microarray to see the transcriptome in collaboration with molecular biology lab, and also perform pathway analysis using bioinformatics tool with our bioinformatics group.
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
Students may be involved in one or more of the following tasks under supervision until he/she is deemed competent to perform them on their own: 1) Cell culture 2) PCR (polymerase chain reaction) 3) Immunohistochemistry 4) Western blot 5) Data analysis and interpretation
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster
- The student will prepare a presentation
- The student will prepare a scientific report
- The student will prepare an abstract
- The student’s name will be mentioned in a future publication
- The student will have the opportunity to present the results together with the supervisor at a conference
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
None
Are there any legal limitations in the student’s involvement
No
Hours
8
Type of students accepted
This project accepts:
- Medical students
Articles
- Alnasser HA; Guan Q; Zhang F; Gleave ME; Nguan CY; Du C. Requirement of clusterin expression for prosurvival autophagy in hypoxic kidney tubular epithelial cells. Am J Physiol Renal Physiol. 2016 Jan 15;310(2):F160-73