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Oncogenesis induced by murine Polyomavirus
Universidad de Buenos Aires
Laboratory of Experimental Pathology. Department of Microbiology, University of Buenos Aires School of Medicine.
Daniel Sordelli, Ph.D. Professor.
Norberto Sanjuan, MD, PhD. Professor
Type of Research Project
- Basic science
What is the background of the project?
Murine Polyomavirus is a small, unenveloped, DNA containing virus able to transform rat cells in vitro and to induce tumors arising in as many as 14 different tissues after inoculation in mice. Some of those tumors strongly resemble neoplasms observed in humans. Thus, it is an excellent experimental model to study cancer pathogenesis with the aim to apply this knew acquired knowledge to the better understanding of tumor development and possible cancer outcomes in patients. The purpose of our work is to study the relationship between viral replication and tumor induction in mice -the virus natural host-, since at a single cell level- both phenomena are mutually excluded.
What is the aim of the project?
The purpose of our work is to study the relationship between viral replication and tumor induction in mice –the virus natural host-, since –at a single cell level- both phenomena are mutually excluded.
What techniques and methods are used?
Classic virology, immunocytochemistry, transmission electron microscopy.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
What are the tasks expected to be accomplished by the student?
Through the study and supervised analysis of slides obtanied from previous experiments performed in our laboratory we aim to promote that the student develops the capacity to infer and propose different possible scenarios which could lead to the obtained results of antigen distribution in different organs, correlated with the presence or absence of viral antigens in tumors occured during the replication phase of the examined samples Simultaneously, the student will be able to apply this recently learned capacities by performing himself/herself peroxidase-antiperoxidase techniques on slides, preparing blocks to study tissues by transmission electron microscopy and ultrastructural immunocytochemistry, performing western blots, cell cultures and virus titration, as well as inoculation of Polyomavirus in newborn mice.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
There is theoretical teaching provided, it is mainly based in the analysis and further discussion of aproximately half a dozen papers related to the subject that will be given to the student during his/her participation in the research.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
The student should have a strong training in basic Medical Sciences (Pathology, Microbiology, Cell Biology, Biochemistry, Molecular Biology. See the web page of the courses of Universidad de Buenos Aires, Facultad de Medicina (www.fmed.uba.ar) Subjects passed: microbiology, pathology
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students
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