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Molecular and Neuroendocrine Mechanisms Controlling Behavior and Stress Responses in Zebrafish (Danio rerio)
Sweden (IFMSA-Sweden) - Uppsala Universtitet, Uppsala
Department of Neuroscience
Prof. Finn Hallbook
Prof. Svante Winberg
Type of Research Project
- Basic science
What is the background of the project?
Identify genes and neuroendocrine mechanisms controlling behavior and stress responses in zebrafish. Behavioral studies include studies on aggressive behavior, risk taking, and learning. Gene expression (by quantifying mRNA concentrations) is studied in separate areas of the zebrafish brain. The project also includes analyses of monoamines (norepinephrine, dopamine and serotonin) and their metabolites in brain tissue. To identify mechanisms controlling the behavioral profile in zebrafish. Interaction between genetic background and environmental cues in forming divergent phenotypes. Behavioral observation of zebrafish, observation is performed manually (e.g. aggressive interaction) or by computerized video tracking systems. Then we will dissect sample brains from zebrafish to isolate specific brain areas. Then extract mRNA from isolated brain areas and synthesize cDNA by reverse transcriptase from this mRNA. Following that we quantify mRNA concentrations from individual brain areas using quantitative polymerase chain reaction (qPCR). Next step is to extract monoamine neurotransmitters (dopamine, norepinephrine, serotonin and their metabolites) from brain tissue and analyze the concentration of these compounds using high performance liquid chromatography (HPLC) with electrochemical detection.
What is the aim of the project?
What techniques and methods are used?
What is the role of the student?
What are the tasks expected to be accomplished by the student?
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months) - Pre-Medical students from the American-British system
- Aggression and monoamines: Effects of sex and social rank in zebrafish (Danio rerio) By: Dahlbom; S. Josefin; Backstrom; Tobias; Lundstedt-Enkel; Katrin; et al. BEHAVIOURAL BRAIN RESEARCH Volume: 228 Issue: 2 Pages: 333-338 Published: MAR 17 2012
- Social modulation of brain monoamine levels in zebrafish By: Teles; Magda C.; Dahlbom; S. Josefin; Winberg; Svante; et al. BEHAVIOURAL BRAIN RESEARCH Volume: 253 Pages: 17-24 Published: SEP 15 2013
- Boldness Predicts Social Status in Zebrafish (Danio rerio) By: Dahlbom; S. Josefin; Lagman; David; Lundstedt-Enkel; Katrin; et al. PLOS ONE Volume: 6 Issue: 8 Article Number: e23565 Published: AUG 17 2011
- Role of brain serotonin in modulating fish behavior By: Winberg; Svante; Thornqvist; Per-Ove CURRENT ZOOLOGY Volume: 62 Issue: 3 Pages: 317-323 Published: JUN 2016
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