Projects
Name
(Craiova) Functional Genomics in severe infections (FUSE)
University
Romania (FASMR) - Univesity of Medicine and Pharmacy Craiova, Craiova
Domain
Genetics
Departement
Human Genomics Laboratory (HGL) ? University of Medicine and Pharmacy of Craiova
Head
Associate Professor Dr.MihaiIoana
Tutor
Associate Professor Dr.MihaiIoana
Languages
English
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No No Yes No Yes No No No
Type of Research Project
- Clinical Project without Laboratory work
What is the background of the project?
Sepsis, defined as the life-threatening organ dysfunction caused by a dysregulated host response to infection, represents a major public health concern. With an estimated worldwide incidence of 19 million cases per year, sepsis is the most frequent cause of death in non-coronary Intensive Care Units (ICUs) of developed countries, with a mortality rate between 28-50% and most likely much higher in poor resource settings. Worldwide the reported incidence of sepsis is increasing, likely reflecting aging populations with more comorbidities, greater recognition, or reimbursement-favorable coding. FUSE will investigate if host defense against sepsis is determined by the genetic background of the host (genome) and the interaction between pathogen colonization and colonizing bacteria and fungi (bacteriome, mycobiome).
What is the aim of the project?
FUSE aims to map the interaction between host genome and bacterial and fungal flora (bacteriome and mycobiome), and its effects on host defense in healthy individuals. Also the project will try to identify the imbalances in these interactions in patients with sepsis and link them to susceptibility to and severity of sepsis.These specific aims will be tackled by the study of genome-microbiome-immune interactions in a group of 500 healthy volunteers and a cohort of 250 sepsis patients.
What techniques and methods are used?
Will be conducted studies in healthy controls and sepsis patients. Activities: 1. enrollment of subjects. 2. transcriptome and genome studies 3. microbiome studies 4. immune response and metabolome evaluation; relationship with genome and microbiome 5. functional validation
What is the role of the student?
What are the tasks expected to be accomplished by the student?
Biological samples processing according to SOPs (Standard operating procedure) - - DNA isolation and purification - Genotyping experiment - Peripheral blood mononuclear cell (PBMC) isolation - Whole blood and peripheral blood mononuclear cell (PBMC) stimulations - Cytokine measurements
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
1
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a presentation
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Skills required: - biological sample handling and storage - pippeting - Measuring volumes - Preparing solutions of known concentration - Preparing reagent solution
Are there any legal limitations in the student’s involvement
No
Hours
6
Type of students accepted
This project accepts:
- Medical students
- Pre-Medical students from the American-British system
Articles
- Singer M; Deutschman CS; Seymour CW; Shankar-Hari M; Annane D; Bauer M; et al. The Third International Consensus Definitions for Sepsis and Septic Shock (Sepsis-3). Jama. 2016;315(8):801-10. - http://jamanetwork.com/journals/jama/fullarticle/2492881
- Angus DC; van der Poll T. Severe sepsis and septic shock. N Engl J Med. 2013;369(9):840-51.- http://www.nejm.org/doi/full/10.1056/NEJMra1208623#t=article