Projects
Name
Effects of the association between maternal diet with protein restriction and exposure to cigarette smoke in lung organogenesis of mice offspring of C57BL /6.
University
Federal University of Ouro Preto- UFOP
Domain
Anaesthesiology
Departement
DECBI ? Departamento de Ci
Head
Prof. Dr. Evandro Marques de Menezes Machado
Tutor
Prof. Dr. Frank Silva Bezerra
Languages
English
Duration
8 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No Yes Yes Yes Yes Yes Yes Yes Yes Yes No
Type of Research Project
- Clinical Project without Laboratory work
What is the background of the project?
Studies have shown that fetal programming based on protein restriction and exposure to cigarette smoke is mainly related to coronary, cardiovascular and renal diseases, but there are no studies in the literature in which the target organ of study to examine the changes resulting from this Programming, be it the lung, as well as all the oxidative stress and inflammatory imbalance caused by such programming.
What is the aim of the project?
The aim of the project is to assess the effects of the association between maternal diet with protein restriction and exposure to cigarette smoke in lung organogenesis of mice offspring of C57BL / 6.
What techniques and methods are used?
In the study will be used 186 mice of the lineage C57BL / 6 from the vivarium of the Laboratory of Experimental Nutrition of the Federal University of Ouro Preto. For mating, 20 females and 10 males will be housed in cages so that 2 females remain for each 1 male for a period of 5 days and after confirmation by vaginal buffer, females will be randomly divided into 2 different groups that will receive two Different isocaloric diets during the gestation period. Experiment: The control group (CG) (n =7) will correspond to the male offspring of mothers fed with standard ration (ration with 19.3% protein content) and exposed to ambient air, according to the group (GC+ FC) (n = 7), the male offspring of mothers fed standard ration (ration with 19.3% protein content) and exposed to HR (12 cig., 3 times a day) during the period Gestational age, the third group (GRPr) (n = 7) will correspond to the male offspring of mothers fed low protein isocaloric ration (ration with 6% protein content), and exposed to ambient air, the fourth group ) (N = 7) will correspond to the male offspring of mothers fed low protein isocaloric ration (6% of the protein content) and exposed to HR (12 cig., 3 times a day) during the gestational period. The third part of each group will be euthanized at the moments: 24 hours after birth, 31 days and 3 months, the bronchoalveolar lavage and lungs will be collected for biochemical, molecular biology and histological analyzes.
What is the role of the student?
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
In the study will be used 186 mice of the lineage C57BL / 6 from the vivarium of the Laboratory of Experimental Nutrition of the Federal University of Ouro Preto. For mating, 20 females and 10 males will be housed in cages so that 2 females remain for each 1 male for a period of 5 days and after confirmation by vaginal buffer, females will be randomly divided into 2 different groups that will receive two Different isocaloric diets during the gestation period. Experiment: The control group (CG) (n =7) will correspond to the male offspring of mothers fed with standard ration (ration with 19.3% protein content) and exposed to ambient air, according to the group (GC+ FC) (n = 7), the male offspring of mothers fed standard ration (ration with 19.3% protein content) and exposed to HR (12 cig., 3 times a day) during the period Gestational age, the third group (GRPr) (n = 7) will correspond to the male offspring of mothers fed low protein isocaloric ration (ration with 6% protein content), and exposed to ambient air, the fourth group ) (N = 7) will correspond to the male offspring of mothers fed low protein isocaloric ration (6% of the protein content) and exposed to HR (12 cig., 3 times a day) during the gestational period. The third part of each group will be euthanized at the moments: 24 hours after birth, 31 days and 3 months, the bronchoalveolar lavage and lungs will be collected for biochemical, molecular biology and histological analyzes.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
1
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Subjects passed: Biochemistry, Physiology and Immunology. Certificate of: Approval in the disciplines of Biochemistry, Physiology and Immunology. You need to be at least in the 4th year of medical studies.
Are there any legal limitations in the student’s involvement
No
Hours
8
Type of students accepted
This project accepts:
- Medical students
Articles
- BARKER; D. J. In utero programming of chronic disease. Clin Sci (Lond); v. 95; n. 2; p. 115-28; Aug 1998. ISSN 0143-5221 (Print)0143-5221.
- HALES; C. N.; BARKER; D. J. The thrifty phenotype hypothesis. Br Med Bull; v. 60; p. 5-20; 2001. ISSN 0007-1420 (Print)0007-1420.
- COSTA; M. R. et al. Grape skin extract-derived polyphenols modify programming-induced renal endowment in prenatal protein-restricted male mouse offspring. Eur J Nutr; Jun 22 2015. ISSN 1436-6207.
- SHAYKHIEV; R.; CRYSTAL; R. G. Innate immunity and chronic obstructive pulmonary disease: a mini-review. Gerontology; v. 59; n. 6; p. 481-9; 2013. ISSN 0304-324x.
- WESTRA; J. W. et al. A modular cell-type focused inflammatory process network model for non-diseased pulmonary tissue. Bioinform Biol Insights; v. 7; p. 167-92; 2013. ISSN 1177-9322.
- ASANO; H. et al. Nicotine- and tar-free cigarette smoke induces cell damage through reactive oxygen species newly generated by PKC-dependent activation of NADPH oxidase. J Pharmacol Sci; v. 118; n. 2; p. 275-87; 2012. ISSN 1347-8613.
- BOUTTEN; A. et al. Oxidative stress targets in pulmonary emphysema: focus on the Nrf2 pathway. Expert Opin Ther Targets; v. 14; n. 3; p. 329-46; Mar 2010. ISSN 1472-8222.
- DROST; E. et al. Oxidative stress and airway inflammation in severe exacerbations of COPD. Thorax; v. 60; n. 4; p. 293-300; Apr 2005. ISSN 0040-6376 (Print)1468-3296 (Electronic).
- DINIZ MF; D. V.; SILVA ME; PEDROSA ML; BEZERRA FS; LIMA WG. Cigarette Smoke Causes Changes in Liver and Spleen of Mice Newborn Exposed During Pregnancy. Journal of Cytology & Histology; v. 2013; 2013-05- 21 2013. ISSN 2157-7099.