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Gene signatures associated with the pathogenesis of the Metabolic Syndrome in mice
Greece (HelMSIC) - University of Crete, Heraklion
Department of Medicine, University of Crete
Professor Giorgos Kontakis
English and Greek
Type of Research Project
- Basic science
What is the background of the project?
The metabolic syndrome (MetS) is a combination of disorders including obesity, insulin resistance, high plasma triglycerides/low HDL cholesterol and high blood pressure that render individuals at increased risk of developing cardiovascular diseases. Several regulatory organs including the liver, the instestine and the adipose tissue are involved in MetS pathogenesis at the gene level. Our goal is to define the role of genes in the pathophysiology of Metabolic Syndrome and how they affect the prediction and evolution of the desease.The aim of this study is to monitor changes in the transcriptome of regulatory organs (liver, white adipose, muscle, pancreas, intestine) of apoE3L.CETP mice used as a model of MetS.MetS is induced by feeding apoE3L.CETP male mice with a diet enriched in fat and cholesterol (HFD) or with a low-fat no-cholesterol diet (LFD) for different time periods (0, 4, 8 and 12 weeks). MetS development is verified on the basis of body weight, total plasma cholesterol and triglycerides and OGTT. RNA is analyzed on Affymetrix Mouse Gene 2.0 ST arrays followed by bioinformatical analysis. Validation of the gene expression changes is performed by reverse transcription qPCR using a Real Time PCR machine.
What is the aim of the project?
What techniques and methods are used?
What is the role of the student?
- If the project includes “lab work”
- the student will take active part in the practical aspect of the project
- If the project is clinical
- the student will take active part in the clinical examination
What are the tasks expected to be accomplished by the student?
Assist in the Lab Work of the project
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
The student needs to have previous experiment with laboratory bench work
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Graduated students (less than 6 months)
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