Identification of Snake venom-derived therapeutic proteins from Bothropsjararaca for cancer treatment.
Brazil (IFMSA-Brazil) - Faculdade de Ciencias da Saude de Barretos Dr. Paulo Prata, Barretos
Molecular Oncology Research Center, Barretos Cancer Hospital ? Barretos, SP, Brazil Rua Antenor Duarte Villela, 1331 Barretos ? SP 14780-400
Rui Reis
Matias Melendez
English, Portuguese, French.
4 weeks
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Type of Research Project
- Basic science
What is the background of the project?
DESCRIPITION: Cancer is one of the greatest health problem in the world, affecting millions of people every year. Head and neck cancer (HNC) is the sixth most common tumor type, and responsible for approximately 350,000 deaths per year. HNC treatment usually combines surgery, chemo- and radiotherapy, with limited success, encouraging studies and developments of more efficient pharmacological agents against this disease. More than a half of approved therapeutic molecules for cancer treatment derived from natural sources, mostly from plant origins. Animal-origin proteins are underexplored potential reservoirs of molecules with potential pharmacological effect. More than 90% of snake venom dry weight is composed of enzymes, such as phospholipases A2, proteases, L-amino acid oxidases, esterases and another non-enzymatic proteins and peptides, with potential pharmacological properties. AIM: The main objectives of this work are (i) to analyze the Bothropsjararaca venom gland transcriptoma, (ii) to clone the coding sequences of interesting therapeutic proteins into expression vectors, and (iii) to analyze their antineoplastic effect for head and neck squamous cell carcinoma, both in vitro and in vivo. USED TECHNIQUES:The first step is to do molecular cloning and other kind of functional assays. The Polymerase Chain Reaction (PCR) is used to increase the amount of genetic material which will be used. At the end it is possible to perform a transcriptome assay.
What is the aim of the project?
What techniques and methods are used?
What is the role of the student?
What are the tasks expected to be accomplished by the student?
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The internship will work mostly as an observership. Furthermore
- the student is expected to have good interaction with the research team
- bench work for molecular cloning experiments. Moreover
- the student will learn how to perform transcriptome
- molecular cloning and other functional assays. Once that procedures are performed by our professional team
- the student will receive theoretical teaching to learn about them and he/she will follow these professional
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
There are no previous skills required. It would be appreciated basic knowledge on microbiology. The work has potential for patenting, in which case the student will not have participation on the property of the patent/process, having participated only in the hands-on execution and not in the design of the project. A scientific report will be demanded after the exchange.
Are there any legal limitations in the student’s involvement

Type of students accepted
This project accepts:
- Medical students
- Other Health sciences students