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Next-Generation-Sequencing analysis of human TCR (T-cell-receptor) repertoires
Germany (bvmd) - Medizinische Hochschule Hannover, Hannover
Hannover Medical School Institute of Immunology, OE 5240, Building I-11, Level 02 Carl-Neuberg-Str. 1, D-30625 Hannover, Germany
Prof. Reinhold Foerster
Prof. Immo Prinz, Dr. Sarina Ravens, Dr. Inga Sandrock
Type of Research Project
- Basic science
What is the background of the project?
We are exploring the development, homeostasis, and function of innate lymphocytes such as NK (natural killer) cells and gamma delta T cells. These cells likely play an important role in the immediate immune response to invading pathogens. The focus of our group is clearly the investigation of gamma delta T cells, in particular of the mechanisms that lead to their differentiation into interferon-gamma or interleukin-17 producing effector cells in humans and in experimental models. To this end, we established protocols for high-throughput TCR (T-cell receptors) sequencing. We investigate the dynamics of gamma delta T cells using a TcrdH2BeGFP reporter knock-in system, which allows us to track gamma delta T cells by means of an intrinsic bright green fluorescence in their nuclei. In parallel, we are generating additional knock-in und knock-out models to study the function of gamma delta T cells and other innate lymphocytes.
What is the aim of the project?
To investigate the dynamics of TCR repertoires in the course of a medical condition, specific subject to be discussed individually depending on interests and timing in the lab.
What techniques and methods are used?
FACS (fluorescence-activated cell scanning) and FACS cell sorting, RNA/ cDNA preparation, Illumina Seq (sequencing), bioinformatical analyses
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
The student will investigate the dynamics of TCR repertoires in the course of a medical condition, specific subject to be discussed individually depending on interests and timing of topics in the lab. In the best of all cases, the student can acquire ethical approvement and actual samples at his / her university that will be analyzed in our pipeline at MHH (Medizinische Hochschule Hannover). Methods involve, but are not restricted to FACS and FACS cell sorting, RNA/ cDNA preparation, Illumina Seq, bioinformatical analyses.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
The student will be expected to participate in regular lab- and departmental seminars.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student’s name will be mentioned in a future publication - No specific outcome is expected
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
The student should be able to perform polymerase chain reaction on his/her own. Subjects passed: Immunology, Molecular Biology. We would like to have a conference call with the student beforehand
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts: - Medical students - Students in biomedical fields
- - Ravens S; Schultze-Florey C; Raha S; Sandrock I; Drenker M; Oberdoerfer L; Reinhardt A; Ravens I; Beck M; Geffers R; von Kaisenberg C; Heuser M; Thol F; Ganser A; Foerster R; Koenecke C*; Prinz I*. Human gamma delta T cells are quickly reconstituted after stem cell transplantation and show adaptive clonal expansion in response to viral infection. Nat Immunol. 2017 Feb 20
- - Kashani E; Foehse L; Raha S; Sandrock I; Oberdoerfer L; Koenecke C; Suerbaum S; Weiss S; Prinz I. A clonotypic Vgamma4Jgamma1/Vdelta5Ddelta2Jdelta1 innate gamma delta T-cell population restricted to the CCR6CD27 subset. Nat Commun. 2015 Mar 13;6:6477.
- - Foehse L; Suffner J; Suhre K; Wahl B; Lindner C; Lee CW; Schmitz S; Haas JD; Lamprecht S; Koenecke C; Bleich A; Haemmerling GJ; Malissen B; Suerbaum S; Foerster R; Prinz I. High TCR diversity ensures optimal function and homeostasis of Foxp3+ regulatory T cells. Eur J Immunol. 2011 Nov;41(11):3101-13
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