Projects
Name
Establish precise medical model of diagnosis and treatment of colorectal clinical studies based on detection of ctDNA liquid biopsy technique
University
China (IFMSA-China) - Southern Medical University, Guangzhou
Domain
Pathophysiology
Departement
Department of Pathophysiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, P.R.China
Head
Yanking Ding
Tutor
Qingling Zhang
Languages
English
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No Yes Yes Yes Yes Yes No Yes Yes Yes Yes
Type of Research Project
- Clinical Project with Laboratory work
What is the background of the project?
Mortality rate of the colorectal Cancer ( CRC) ranks fourth in cancer , according to the United States National Cancer Institute(NCI) statistics. In China, the CRC incidence and mortality is high among malignant cancers. Lack of obvious early symptoms and inconvenient disease screening method make it very hard to diagnose and there is no simple and reliable method to monitor progression. The monitor of CRC is still supported by imaging and endoscopic biopsy. To establish a simple, strong control of screening methods may improve colorectal cancer detection rates, reduce detection time and establish dynamic observation systems of cancer progressive monitoring. Biopsy of the liquid (Liquid biopsy) not only solve the above issues ,but also detect cancer ahead of time. The ctDNA of tumor provides unprecedented vision of comprehensive understanding of tumor characteristics and precised drug delivery. 1.To establish a sensitve blood test technology for colorectal cancer. 2.Using ctDNA liquid biopsy technology to achieve the early screening , progressive monitoring and liver metastasis predicating of colorectal cancer. 3.Publish 1-3 clinical study manuscriptes aboutliquid biopsy. Liquid biopsies have been made possible by advances in sequencing the human genome since scientists and researchers can now detect genetic mutations of cancers. Researchers have found that unique cancer mutations can show up in microscopic fragments of DNA in a patient's blood. Tumor-related circulating-free DNA, RNA or proteins are released by tumor cells and circulate in the blood of cancer patients. Therefore, assays using these molecules can be used for early tumor detection, monitoring or detection of resistance mutations. Digital PCR technology Digital PCR builds on traditional PCR amplification and fluorescent-probe?based detection methods to provide highly sensitive absolute quantification of nucleic acids without the need for standard curves. In the Droplet Digital
What is the aim of the project?
1.To establish a sensitve blood test technology for colorectal cancer. 2.Using ctDNA liquid biopsy technology to achieve the early screening , progressive monitoring and liver metastasis predicating of colorectal cancer. 3.Publish 1-3 clinical study manuscriptes aboutliquid biopsy.
What techniques and methods are used?
Liquid biopsies have been made possible by advances in sequencing the human genome since scientists and researchers can now detect genetic mutations of cancers. Researchers have found that unique cancer mutations can show up in microscopic fragments of DNA in a patient's blood. Tumor-related circulating-free DNA, RNA or proteins are released by tumor cells and circulate in the blood of cancer patients. Therefore, assays using these molecules can be used for early tumor detection, monitoring or detection of resistance mutations. Digital PCR technology Digital PCR builds on traditional PCR amplification and fluorescent-probe–based detection methods to provide highly sensitive absolute quantification of nucleic acids without the need for standard curves. In the Droplet Digital™ PCR System, a PCR sample is partitioned into 20,000 droplets. After amplification, droplets containing target sequence are detected by fluorescence and scored as positive, and droplets without fluorescence are scored as negative.
What is the role of the student?
- The student will mainly observe
- The tasks of the student will be performed on his/her own
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will be assigned to do real-time PCR on his or her own to detect gene mutation by he sample of circulating DNA from the blood of the patient. Apart from that, the student will need to go to the nan fang hospital to obtain the blood sample of the patient under supervised. Sometimes, the student may need to prepare facility for other members of this laboratory and do some cleaning when he or she is on duty.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student’s name will be mentioned in a future publication
- The student will have the opportunity to present the results together with the supervisor at a conference
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
none
Are there any legal limitations in the student’s involvement
No
Hours
7
Type of students accepted
This project accepts:
- Medical students
Articles