Projects
Name
Mitochondria as a therapeutic agent in Immune-Regulation, Tissue Repair and Fertility
University
Universidad San Francisco de Quito
Domain
Immunology
Departement
Molecular biology laboratory of the USFQ
Head
Michelle Grunauer MD Ph.D.
Tutor
Andrés Caicedo
Languages
Required: English Accepted: Spanish
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
Yes Yes Yes Yes Yes Yes No No Yes Yes Yes Yes
Type of Research Project
- Basic science
What is the background of the project?
Mitochondria have a fundamental role in growth, differentiation and survival of cells beyond sustain their energetics. Diseases, tissue damage and aging challenge the cell and its mitochondria affecting their integrity, function and homeostasis. Naturally, Mesenchymal Stem Cells (MSCs), commonly used for cell therapy, are able to transmit their mitochondria and other factors to harmed cells, restoring the loss of function and regulating the immune response. Inspired by this natural process we develop a technique named MitoCeption based on the artificial transfer of mitochondria from one cell to another. Our goal is to optimize this method and apply it in regenerative medicine Our objective is to transfer mitochondria artificially to immune cells to reprogram their phenotype to induce immune regulation. Repair the damaged mitochondria of an oocyte by introducing healthy copies, and finally increase the migration and proliferation of immune cells.
What is the aim of the project?
Our objective is to transfer mitochondria artificially to immune cells to reprogram their phenotype to induce immune regulation. Repair the damaged mitochondria of an oocyte by introducing healthy copies, and finally increase the migration and proliferation of immune cells.
What techniques and methods are used?
Cell Culture and Molecular analysis
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
Follow up of the project leader, learn, replicate and analyze results. Knowledge of the project background, hypothesis and what are the approaches to verify or reject it.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Seminars by the School of Medicine
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster
- The student will prepare a scientific report
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Basic knowledge in laboratory practices, cell culture and molecular analysis like qPCR will be highly appreciated.
Are there any legal limitations in the student’s involvement
No
As an special remark: Join the poster and if a significant amount of work has been provided, paper.
Hours
6
Type of students accepted
This project accepts:
- Medical students
- Graduated students (less than 6 months)
Articles
- http://www.nature.com/articles/srep09073