Projects
Name
Changes of brain proteome composition and thermodynamic properties in neurodegenerative disorders relation of proteome exothermic process with the mechanism of plaque formation
University
Bulgaria (AMSB) - Medical University of Sofia, Sofia
Domain
Biophysics and Medical Physics
Departement
Department of Medical Physics and Biophysics, Medical University ? Sofia
Head
professor Boris Tenchov, PhD, DSc
Tutor
Silviya Abarova, Lyubomir Traikov
Languages
English
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
Yes Yes Yes Yes Yes Yes No No Yes Yes Yes Yes
Type of Research Project
- Basic science
What is the background of the project?
The main objective of this project is to identify changes in the composition of the brain proteome caused by neurodegenerative disorders (drug induced dementia in experimental animals) with a focus on identifying the proteins responsible for the thermal transitions in the proteome observed by differential scanning calorimetry (DSC). The cerebrospinal fluid (CSF) of healthy and demented animals will be examined by DSC in order to clarify whether the changes in the proteomic composition and the DSC profiles adequately reflect the development of the neurodegenerative process. Part of these studies will be performed on the CSF of patients suffering from Alzheimer's disease in advanced stages, in order to clarify the potential of the DSC as a method for diagnosis. Also, it is important to clarify the ability of biologically active substances with an anticipated neuroprotective capacity to influence the proteomic composition and CSF of demented animals. Obtaining new knowledge concerning the neurodegenerative disorders, which will contribute to a deeper understanding of the nature of these diseases. 1. Preparation of tissue homogenates and supernatants from brain and liver 2. Isolation of cerebrospinal fluid from Cisterna Magna of rats 3. Preparation of blood serum / plasma by puncture of the tail vein (vena caudalis) 4. Obtaining cerebrospinal liquid from patients with Alzheimer's disease 5. Obtaining blood samples of patients with Alzheimer's disease 6. Fractionation of brain supernatants and cerebrospinal fluid 7. Differential scanning calorimetry (DSC) (Nano DSC, TA Instruments, USA). 8. Dynamic light scattering 9. Other methods. Isothermal titration calorimetry (ITC), fluorescence spectroscopy, densitometry.
What is the aim of the project?
Obtaining new knowledge concerning the neurodegenerative disorders, which will contribute to a deeper understanding of the nature of these diseases.
What techniques and methods are used?
1. Preparation of tissue homogenates and supernatants from brain and liver 2. Isolation of cerebrospinal fluid from Cisterna Magna of rats 3. Preparation of blood serum / plasma by puncture of the tail vein (vena caudalis) 4. Obtaining cerebrospinal liquid from patients with Alzheimer's disease 5. Obtaining blood samples of patients with Alzheimer's disease 6. Fractionation of brain supernatants and cerebrospinal fluid 7. Differential scanning calorimetry (DSC) (Nano DSC, TA Instruments, USA). 8. Dynamic light scattering 9. Other methods. Isothermal titration calorimetry (ITC), fluorescence spectroscopy, densitometry.
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
Working with laboratory animals. Establish experimental model of neurodegenerative disorders of AD type and treatment with biologically active compounds. Preparation of tissue homogenates and supernatants from brain and liver. Isolation of cerebrospinal fluid from Cisterna Magna of rats. Preparation of blood serum / plasma by puncture of the tail vein (vena caudalis). Obtaining cerebrospinal liquid from patients with Alzheimer's disease. Fractionation of brain supernatants and cerebrospinal fluid. Working with methods for study of tissue samples and body fluids: Differential scanning calorimetry (DSC) (Nano DSC, TA Instruments, USA); Dynamic light scattering; Isothermal titration calorimetry (ITC); Fluorescence spectroscopy; Densitometry.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
We will provide the student with preliminary readings a few weeks before his arrival.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster
- The student will prepare a scientific report
- The student will have the opportunity to present the results together with the supervisor at a conference
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Highly motivated to do laboratory experimental work Interest in investigation of neurodegenerative diseases Basic knowledge of physics, biology and chemistry
Are there any legal limitations in the student’s involvement
No
Hours
6
Type of students accepted
This project accepts:
- Medical students
- Pre-Medical students from the American-British system
Articles
- Calorimetry Outside the Box: A New Window into the Plasma Proteome Nichola C. Garbett;* James J. Miller;y Alfred B. Jenson;* and Jonathan B. Chaires
- Differential Scanning Calorimetry of Gliomas: A New Tool in Brain Cancer Diagnostics? Alexis A. Chagovetz; BS; Colette Quinn; PhD*; Neil Damarse; PhD; Lee D. Hansen; PhD; Alexander M. Chagovetz; PhD; Randy L. Jensen; MD; PhD
- A NEW APPROACH FOR INVESTIGATING DRUG-INDUCED NEURODEGENERATIVE DISORDERS IN MICE AND METHODS FOR THEIR PREVENTION BASED ON DIFFERENTIAL SCANNING CALORIMETRY (DSC) Boris Tenchov; Silviya Abarova; Rumiana Koynova; Lubomir Traikov; Stela Dragomanova; LyubkaTancheva
- Reflection of changes in in membrane constituents in various regions of Alzheimer brains to Differential scanning calorimetry E. Sofic; M. Gotz; L. Frolich; R. Burger; S. Heckers; P. Riederer; K. Jellinger; H. Beckmann