Projects
Name
Exploring the effects of some hormones with antioxidant potential on induction and complications of experimentally induced type 2 diabetes mellitus.
University
Bulgaria (AMSB) - Medical faculty of Trakia University, Stara Zagora
Domain
Physiology
Departement
Department of Physiology, Pathophysiology and Pharmacology, Medical Faculty, Trakia University, Stara Zagora, Bulgaria
Head
Prof. Dr. Anna Tolekova, MD, Ph
Tutor
Prof. Dr. Anna Tolekova, MD, PhD Assoc. Prof. Dr. Maria Ganeva, MD, PhD
Languages
English , Russian
Duration
4 weeks
Availability
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
Yes No No No No Yes Yes No No No No No
Type of Research Project
- Basic science
What is the background of the project?
Diabetes mellitus is becoming one of the most significant diseases in the world. This process is accompanied by an increase in the incidence of complications, especially with regard to micro- and macro vascular pathology and disturbances of visceral smooth muscle contractility. These prerequisites determine the growing interest of scientists from all over the world in the issues related to B-cell disorders, insulin resistance, and the factors that determine the onset and progression of vascular and smooth muscle complications. The efforts of a number of scientific teams have focused on the creation and use of experimental models to allow investigation of the effect of different substances on the appearance and progression of the disease. To investigate the effects of ghrelin, melatonin and vitamin D on an experimental model of type 2 diabetes mellitus and its complications - smooth muscle dysfunction and diabetic nephropathy as well as changes in Renin – Angiotensin System (RAS). To investigate adverse reactions induced by RAS-modifying drugs and related biologically active substances and the associated changes in some pro-inflammatory cytokines in the context of a post marketing study in patients, hospitalized in the University Hospital "Prof. Dr. Stoyan Kirkovich”, Stara Zagora.
What is the aim of the project?
To investigate the effects of ghrelin, melatonin and vitamin D on an experimental model of type 2 diabetes mellitus and its complications - smooth muscle dysfunction and diabetic nephropathy as well as changes in Renin – Angiotensin System (RAS). To investigate adverse reactions induced by RAS-modifying drugs and related biologically active substances and the associated changes in some pro-inflammatory cytokines in the context of a post marketing study in patients, hospitalized in the University Hospital "Prof. Dr. Stoyan Kirkovich”, Stara Zagora.
What techniques and methods are used?
I. Experimental studies on laboratory animals (rats). 1 stage. In vivo Induction of type 2 diabetes and its verification. Treatment of some experimental groups with ghrelin, melatonin and vitamin D only and monitoring their effects during the experimental period. Induction of diabetes mellitus in animals treated with the respective hormones before or after theinduction. 2 stage. In vitro Isolated tissues method: Analysis and comparison of individual parameters of smooth muscle activity: amplitude, frequency, integral muscle force, speed of contraction and relaxation of strips from urinary bladder and gastrointestinal tract. Determination of glucose level, glycated hemoglobin, lipid profile, C reactive protein, plasma renin activity, plasma levels of insulin, ghrelin and leptin 3 stage Graphical analysis of the obtained smooth muscle contraction curves by a specific software 4 stage. Statistical processing. Preparing articles and presentations, reflecting and discussing the results II. Clinical and pharmacological studies Identification of adverse reactions induced by RAS-modifying medications and related biologically active substances Measurement of serum levels of IL-17 and IL-23 in the blood of such patients
What is the role of the student?
- The student will mainly observe
- The student will observe the practical experiments but will be highly involved in the analysis of the results
What are the tasks expected to be accomplished by the student?
Participation in the induction of the experimental diabetes type 2: daily preparation of fructose solutions for drinking and low dose streptozotocin injections. Treatment of the experimental animals with the potential anti-oxidants and monitoring morpho-functional changes in the animals during the experimental period Participation in the organ preparations and study of the smooth muscle function by the isolated tissue system Collecting blood for laboratory analysis Participation in the processing of the primary data, obtained from the smooth muscle contractions – filtering, transformation and translation of the contraction curves. Participation in the analysis and the statistical processing.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Preliminary materials concerning the area of study will be provided as well as a full explanation of each stage of the study process, in which the student participates.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
Working with experimental animals: fixation, injection and blood collection Working with laboratory equipment Basic knowledge in physiology and pathophysiology
Are there any legal limitations in the student’s involvement
No
Hours
6
Type of students accepted
This project accepts:
- Medical students
- Pre-Medical students from the American-British system
Articles
- Chatzigeorgiou A; A. Halapas; K. Kalafatakis; E.Kamper. The use of animal models in the study of diabetes mellitus.In Vivo. 2009; 23(2): 245-58.
- Wang C.C. et al. Diabetes- induced Alterations of Biomechanical Properties of Urinary Bladder Wall in Rats. Urology. 2009; 73(4): 911-915.
- Liu G.; F. Daneshgari. Temporal diabetes- and diuresis-induced remodeling of the urinary bladder in the rat. Am J Physiol. 2006; 291: R837-43
- Yavus O.; M. Cam; N. Bukan; A. Guven; F. Silan. Protective effect of Melatonin on β-cell damage in streptozotocin-induced diabetes in rats. Acta histochemica. 2003; 105(3): 261-266.
- Irako T; T. Akamizu; H. Hosoda; H. Iwakura; H. Ariyasu; K. Tojo; N. Tajima; K. Kangawa. Ghrelin prevents development of diabetes at adult age in streptozotocin-treated newborn rats.Diabetologia. 2006;49(6):1264-73.
- Pulkkinen; L.; Ukkola; O.; Kolehmainen; M.; Uusitupa; M. and Corporation; H.P. (2010) Ghrelin in diabetes and metabolic syndrome. Available at: http://www.hindawi.com/journals/ijpep/2010/248948/abs/
- Campos LA; Cipolla-Neto J; Amaral FG; Michelini LC; Bader M; Baltatu OC. The Angiotensin-melatonin axis. Int J Hypertens. 2013;2013:521783
- Han JH; Chang IH; Myung SC; Lee MY; Kim WY; Lee SY; Lee SY; Lee SW; Kim KD. A Novel Pathway Underlying the Inhibitory Effects of Melatonin on Isolated Rat Urinary Bladder Contraction. Korean J Physiol Pharmacol. 2012;16(1):37-42
- Vangoitsenhoven R; Wolden-Kirk H; Lemaire K; Verstuyf A; Verlinden L; Yamamoto Y; Kato S; Van Lommel L; Schuit F; Van der Schueren B; Mathieu C; Overbergh L.Effect of a transcriptional inactive or absent vitamin D receptor on beta-cell function and glucose homeostasis in mice. J Steroid Biochem Mol Biol. 2016 Feb 11. pii: S0960-0760(16)30024-3.