Functional cytolitic analysis of natural killer cells against pancreatic cancer cell lines.
Portugal (PorMSIC) - University of Coimbra, Coimbra
General Pathology Laboratory, Faculty of Medicine, University of Coimbra, Portugal and Immunology Institute, Faculty of Medicine, University of Coimbra, Portugal.
Anabela Mota-Pinto (PhD, Full Professor) and Manuel Santos Rosa (PhD, Full Professor).
Rui Gradiz (Assistant Professor, MD, PhD), Dr. Paulo Rodrigues Santos (Lecturer)
French, Spanish
4 weeks
Cities/Months Jan Feb Mar Apr May Jun Jul Augt Sep Oct Nov Dec
No No No No No No Yes No No No No No
Type of Research Project
- Basic science
What is the background of the project?
Pancreatic cancer, the fourth leading cause of cancer worldwide, is a major cause of death, with a high mortality rate, that could be explained by the late diagnosis and the extremely aggressive characteristic of this type of cancer. This is a tumor resistant to surgery, chemotherapy and radiotherapy with a five-year survival rate of less than 5 percent. MIA PaCa-2, PANC-1 and Hs766T are human pancreatic duct adenocarcinoma cell lines, a cancer that represents about 90 percent of all pancreatic tumors. Natural killer cells (or NK cells) are cytotoxic lymphocytes effectors of the innate immune system. The expression and function of both inhibitory and activating receptors are involved in the molecular regulation of innate immunity in malignant setting. NK cells are potential tools for cancer therapy due to cytotoxic ability enhanced by antibodies and the possibility of ex vivo expansion and adoptive transfer.
What is the aim of the project?
We aim to determine the cytolytic activity of allogenic NK cells against pancreatic cancer cell lines MIA PaCa-2, PANC-1 and Hs766T.
What techniques and methods are used?
Cell Culture and Flow cytometry
What is the role of the student?
- The student will observe the practical experiments but will be highly involved in the analysis of the results
- The tasks will be done under supervision
What are the tasks expected to be accomplished by the student?
The student will take an active part in research and we expect him to fulfill the tasks and time planning assigned to him. In the tasks projected for the four weeks, we expect to determine the cytolytic activity of allogenic NK cells, obtained from ten healthy human donors, against pancreatic cancer cell lines MIA PaCa-2, PANC-1 and Hs766T, and to obtain information regarding the expression of the cell receptors and cytokines production. The student will perform by himself cell cultures and flow cytometry techniques.
Will there be any theoretical teaching provided (preliminary readings, lectures, courses, seminars etc)
Yes, Preliminary readings, lectures or seminars.
What is expected from the student at the end of the research exchange? What will be the general outcome of the student?
- The student will prepare a poster
- The student will prepare a presentation
What skills are required of the student? Is there any special knowledge or a certain level of studies needed?
No requirements.
Are there any legal limitations in the student’s involvement
Type of students accepted
This project accepts:
- Medical students
- 1. Siegel RL; Miller KD; Jemal A. Cancer statistics; 2015. CA Cancer J Clin 2015. 65:5-29.
- 2. Gradiz; Rui; Silva; Henriqueta C; Carvalho; Lina; Botelho; Maria F; Mota-Pinto; Anabela. 2016. MIA PaCa-2 and PANC-1 ? pancreas ductal adenocarcinoma cell lines with neuroendocrine differentiation and somatostatin receptors; Scientific Reports 6; 21648: 1 - 14.
- 3. Deer EL; Gonzalez-Hernandez J; Coursen JD; Shea JE; Ngatia J; Scaife CL; et al. Phenotype and genotype of pancreatic cancer cell lines. Pancreas 2010; 39(4):425-435.
- 4. Cheng M; Chen Y; Xiao W; Sun R; Tian Z. NK cell-based immunotherapy for malignant diseases. Cell Mol Immunol 2013;10:230-252.